RPN14/YGL004C Summary Help

Standard Name RPN14 1
Systematic Name YGL004C
Feature Type ORF, Verified
Description Assembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p (1, 2, 3, 4, 5, 6)
Name Description Regulatory Particle Non-ATPase 1
Chromosomal Location
ChrVII:491956 to 490703 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RPN14 GO evidence and references
  View Computational GO annotations for RPN14
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
86 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 23
  • Affinity Capture-Western: 31
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 8
  • Phenotypic Enhancement: 4
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 417
Molecular Weight (Da) 46,383
Isoelectric Point (pI) 4.94
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:491956 to 490703 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1254 491956..490703 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002972
References cited on this page View Complete Literature Guide for RPN14
1) Samanta MP and Liang S  (2003) Predicting protein functions from redundancies in large-scale protein interaction networks. Proc Natl Acad Sci U S A 100(22):12579-83
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Seong KM, et al.  (2007) Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome. FEBS Lett 581(13):2567-73
4) Roelofs J, et al.  (2009) Chaperone-mediated pathway of proteasome regulatory particle assembly. Nature 459(7248):861-5
5) Saeki Y, et al.  (2009) Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle. Cell 137(5):900-13
6) Funakoshi M, et al.  (2009) Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell 137(5):887-99