Standard Name	KRE28 1
Systematic Name	YDR532C
Feature Type	ORF, Verified
Description	Subunit of a kinetochore-microtubule binding complex with Spc105p that bridges centromeric heterochromatin and kinetochore MAPs and motors, and is also required for sister chromatid bi-orientation and kinetochore binding of SAC components (1, 2)

Chromosomal Location	ChrIV:1500553 to 1499396 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for KRE28
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	biological_process unknown (ND)
Cellular Component
Manually curated	chromosome, centromeric region (IDA) condensed nuclear chromosome kinetochore (IDA)
High-throughput	colocalizes_with spindle pole body (IDA)

Classical genetics
null	chromosome/plasmid maintenance: abnormal inviable resistance to tellurite: decreased
Large-scale survey
null	bud morphology: abnormal cell size: increased proton accumulation: increased competitive fitness: decreased endocytosis: decreased heat sensitivity: increased hyperosmotic stress resistance: decreased metal resistance: increased resistance to CTBT: decreased resistance to hydroxyurea: decreased resistance to amitrole: decreased resistance to benomyl: decreased resistance to wortmannin: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to methyl methanesulfonate: decreased resistance to hygromycin B: decreased resistance to spermine: decreased resistance to cycloheximide: decreased resistance to calcium dichloride: decreased resistance to caffeine: decreased resistance to doxorubicin: decreased resistance to sirolimus: decreased telomere length: decreased toxin resistance: decreased utilization of phosphorus source: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	69 total interaction(s) for 57 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-Western: 4 Co-crystal Structure: 1 Co-fractionation: 1 Co-localization: 1 Two-hybrid: 7
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 24 Positive Genetic: 12 Synthetic Growth Defect: 5 Synthetic Lethality: 7 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:1500553 to 1499396 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1158 1500553..1499396 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002940