GRH1/YDR517W Summary Help

Standard Name GRH1 1, 2
Systematic Name YDR517W
Feature Type ORF, Verified
Description Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress (3, 4, 5)
Name Description GRasp65 (Golgi reassembly stacking protein of 65kD) Homolog
Chromosomal Location
ChrIV:1477239 to 1478357 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GRH1 GO evidence and references
  View Computational GO annotations for GRH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
48 total interaction(s) for 32 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • PCA: 5

Genetic Interactions
  • Negative Genetic: 20
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 372
Molecular Weight (Da) 41,119
Isoelectric Point (pI) 4.95
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1477239 to 1478357 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1119 1477239..1478357 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002925
References cited on this page View Complete Literature Guide for GRH1
1) Barr F A  (2003) personal communication
2) Shorter J and Warren G  (2002) Golgi architecture and inheritance. Annu Rev Cell Dev Biol 18:379-420
3) Norman TC, et al.  (1999) Genetic selection of peptide inhibitors of biological pathways. Science 285(5427):591-5
4) Behnia R, et al.  (2007) The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic. J Cell Biol 176(3):255-61
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76