SEC20/YDR498C Summary Help

Standard Name SEC20 1
Systematic Name YDR498C
Feature Type ORF, Verified
Description Membrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to the endoplasmic reticulum (ER); required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p (2, 3, 4)
Name Description SECretory 5
Chromosomal Location
ChrIV:1446994 to 1445843 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SEC20 GO evidence and references
  View Computational GO annotations for SEC20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
repressible
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
63 total interaction(s) for 23 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 15
  • Co-fractionation: 1
  • Co-localization: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 8
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 383
Molecular Weight (Da) 43,882
Isoelectric Point (pI) 5.92
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1446994 to 1445843 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1152 1446994..1445843 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002906
References cited on this page View Complete Literature Guide for SEC20
1) Novick P, et al.  (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21(1):205-15
2) Pelham HR  (1999) SNAREs and the secretory pathway-lessons from yeast. Exp Cell Res 247(1):1-8
3) Schleip I, et al.  (2001) The yeast SEC20 gene is required for N- and O-glycosylation in the Golgi. Evidence that impaired glycosylation does not correlate with the secretory defect. J Biol Chem 276(31):28751-8
4) Tripathi A, et al.  (2009) Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16(2):114-23
5) Novick P and Schekman R  (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76(4):1858-62