Standard Name	PUF6
Systematic Name	YDR496C
Feature Type	ORF, Verified
Description	Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis (1, 2)
Name Description	PUmilio-homology domain Family

Chromosomal Location	ChrIV:1443403 to 1441433 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for PUF6
Molecular Function
Manually curated	mRNA 3'-UTR binding (IDA) translation repressor activity, nucleic acid binding (IDA)
Biological Process
Manually curated	negative regulation of translation (IDA) ribosomal large subunit biogenesis (IMP)
Cellular Component
Manually curated	large ribosomal subunit (IDA)
High-throughput	nucleolus (IDA) nucleus (IDA)

Classical genetics
null	cold sensitivity: increased nuclear export: decreased 60S ribosomal subunit accumulation: decreased
Large-scale survey
null	competitive fitness: decreased haploinsufficient heat sensitivity: increased hyperosmotic stress resistance: decreased resistance to quinine: decreased resistance to sirolimus: decreased resistance to mycophenolic acid: increased resistance to camptothecin: increased resistance to bleomycin: increased resistance to cycloheximide: decreased resistance to amitrole: decreased resistance to streptomycin: decreased resistance to tunicamycin: decreased resistance to tenovin-6: decreased resistance to fluconazole: decreased stress resistance: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	224 total interaction(s) for 154 unique genes/features.
Physical Interactions	Affinity Capture-MS: 144 Affinity Capture-RNA: 4 Affinity Capture-Western: 4 Biochemical Activity: 1 Co-fractionation: 1 Reconstituted Complex: 1
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 39 Phenotypic Enhancement: 2 Positive Genetic: 20 Synthetic Growth Defect: 4 Synthetic Lethality: 2 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:1443403 to 1441433 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1971 1443403..1441433 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002904