PKH1/YDR490C Summary Help

Standard Name PKH1 1
Systematic Name YDR490C
Feature Type ORF, Verified
Description Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Pkb-activating Kinase Homolog 1
Chromosomal Location
ChrIV:1434268 to 1431968 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All PKH1 GO evidence and references
  View Computational GO annotations for PKH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
126 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-Western: 4
  • Biochemical Activity: 12
  • PCA: 2
  • Reconstituted Complex: 10
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 7
  • Dosage Rescue: 1
  • Negative Genetic: 32
  • Phenotypic Enhancement: 1
  • Positive Genetic: 13
  • Synthetic Growth Defect: 19
  • Synthetic Lethality: 4
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 766
Molecular Weight (Da) 86,252
Isoelectric Point (pI) 10.02
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1434268 to 1431968 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2301 1434268..1431968 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002898
References cited on this page View Complete Literature Guide for PKH1
1) Casamayor A, et al.  (1999) Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Curr Biol 9(4):186-97
2) Sun Y, et al.  (2000) Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast. Mol Cell Biol 20(12):4411-9
3) Friant S, et al.  (2001) Sphingoid base signaling via Pkh kinases is required for endocytosis in yeast. EMBO J 20(23):6783-92
4) Roelants FM, et al.  (2002) Pkh1 and Pkh2 differentially phosphorylate and activate Ypk1 and Ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Mol Biol Cell 13(9):3005-28
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61