SLD5/YDR489W Summary Help

Standard Name SLD5
Systematic Name YDR489W
Alias CDC105
Feature Type ORF, Verified
Description Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery (1, 2 and see Summary Paragraph)
Name Description Synthetic Lethality with Dpb11-1
Chromosomal Location
ChrIV:1431012 to 1431896 | ORF Map | GBrowse
Gene Ontology Annotations All SLD5 GO evidence and references
  View Computational GO annotations for SLD5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
121 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 24
  • Affinity Capture-Western: 57
  • Biochemical Activity: 1
  • Co-purification: 1
  • Reconstituted Complex: 18
  • Two-hybrid: 13

Genetic Interactions
  • Dosage Rescue: 2
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2

Expression Summary
Length (a.a.) 294
Molecular Weight (Da) 33,947
Isoelectric Point (pI) 4.53
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1431012 to 1431896 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..885 1431012..1431896 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002897

The GINS complex (named for Go, Ichi, Nii, and San for five, one, two, and three in Japanese) is composed of four subunits, encoded by SLD5, PSF1, PSF2, and PSF3. In S. cerevisiae, it was first identified by genetic and biochemical methods to determine factors interacting with Sld5p. Genetic interactions between these four genes also suggest that they act together (1). The GINS complex was independently isolated in a large scale screen for cell cycle defects (2). A similar complex is found in Xenopus and has a ring-like structure (3). In yeast, all four genes are essential and cells defective in SLD5, PSF1, or PSF2 are impaired in their ability to replicate DNA (1, 2). The complex localizes to origins of DNA replication (1, 2) and Sld5p was previously implicated as functioning in DNA replication due to its genetic interaction with DPB11 (4). Additional genetic and biochemical interactions of the GINS complex with Dpb11p, Dpb2p, and Sld3p suggest that it functions in some way at the replication fork during DNA synthesis (1).

Last updated: 2005-12-02 Contact SGD

References cited on this page View Complete Literature Guide for SLD5
1) Takayama Y, et al.  (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17(9):1153-65
2) Kanemaki M, et al.  (2003) Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature 423(6941):720-4
3) Kubota Y, et al.  (2003) A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication. Genes Dev 17(9):1141-52
4) Kamimura Y, et al.  (1998) Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol 18(10):6102-9