VPS52/YDR484W Summary Help

Standard Name VPS52 1, 2
Systematic Name YDR484W
Alias SAC2 3
Feature Type ORF, Verified
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p (1, 4, 5, 6)
Name Description Vacuolar Protein Sorting
Chromosomal Location
ChrIV:1422763 to 1424688 | ORF Map | GBrowse
Gbrowse
Genetic position: 314 cM
Gene Ontology Annotations All VPS52 GO evidence and references
  View Computational GO annotations for VPS52
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
235 total interaction(s) for 127 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 26
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 16
  • Co-fractionation: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 85
  • Phenotypic Enhancement: 20
  • Phenotypic Suppression: 40
  • Positive Genetic: 15
  • Synthetic Growth Defect: 8
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 12
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 641
Molecular Weight (Da) 74,332
Isoelectric Point (pI) 5.54
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1422763 to 1424688 | ORF Map | GBrowse
SGD ORF map
Genetic position: 314 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1926 1422763..1424688 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002892
References cited on this page View Complete Literature Guide for VPS52
1) Conibear E and Stevens TH  (2000) Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell 11(1):305-23
2) Novick, P., et al.  (1989) Personal Communication, Mortimer Map Edition 10
3) Kolling R, et al.  (1994) Nucleotide sequence of the SAC2 gene of Saccharomyces cerevisiae. Yeast 10(9):1211-6
4) Winsor B and Schiebel E  (1997) Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast 13(5):399-434
5) Siniossoglou S and Pelham HR  (2002) Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem 277(50):48318-24
6) Dotiwala F, et al.  (2013) DNA damage checkpoint triggers autophagy to regulate the initiation of anaphase. Proc Natl Acad Sci U S A 110(1):E41-9