Standard Name	SSN2 1
Systematic Name	YDR443C
Alias	NUT8 2 , SCA1 , SRB9 , UME2 , RYE3 , MED13 3
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation (1, 4, 5)
Name Description	Suppressor of SNf1 1

Chromosomal Location	ChrIV:1349938 to 1345676 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for SSN2
Molecular Function
Manually curated	RNA polymerase II transcription coactivator activity (IDA) RNA polymerase II transcription factor recruiting transcription factor activity (IMP)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter (IDA) positive regulation of transcription from RNA polymerase II promoter by galactose (IMP)
Cellular Component
Manually curated	mediator complex (IDA)

Classical genetics
null	chromosome/plasmid maintenance: abnormal Ptr2p-GFP accumulation: increased
unspecified	flocculation: increased heat sensitivity: increased survival rate in stationary phase: decreased
Large-scale survey
null	Protein A-nonstop reporter mRNA accumulation: increased competitive fitness: decreased dessication resistance: decreased heat sensitivity: increased nutrient utilization: increased resistance to selenomethionine: increased resistance to nickel sulfate: increased resistance to 5-fluorouracil: decreased resistance to mefloquine: decreased resistance to benzo[a]pyrene: decreased resistance to cycloheximide: decreased sporulation: decreased sporulation efficiency: decreased stress resistance: decreased telomere length: increased toxin resistance: decreased transposable element transposition: decreased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	260 total interaction(s) for 186 unique genes/features.
Physical Interactions	Affinity Capture-MS: 49 Affinity Capture-RNA: 1 Affinity Capture-Western: 8 Biochemical Activity: 2 Two-hybrid: 9
Genetic Interactions	Dosage Rescue: 4 Negative Genetic: 101 Phenotypic Enhancement: 8 Phenotypic Suppression: 5 Positive Genetic: 43 Synthetic Growth Defect: 16 Synthetic Lethality: 8 Synthetic Rescue: 6
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:1349938 to 1345676 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..4263 1349938..1345676 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002851