NPL3/YDR432W Summary Help

NPL3 BASIC INFORMATION

Standard Name NPL3 1
Systematic Name YDR432W
Alias MTR13 2 , MTS1 3 , NOP3 4 , NAB1 5
Feature Type ORF, Verified
Description RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm (6, 7, 8, 9, 10)
Name Description Nuclear Protein Localization 1
GO Annotations All NPL3 GO evidence and references
    View Computational GO annotations for NPL3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant Phenotype All NPL3 Phenotype details and references
Classical genetics
conditional
null
Large-scale survey
null
overexpression
Interactions NPL3 All interactions details and references
294 total interaction(s) for 206 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 78
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 23
  • Biochemical Activity: 13
  • Co-localization: 3
  • Reconstituted Complex: 7
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 68
  • Phenotypic Suppression: 22
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 27
  • Synthetic Rescue: 5

Sequence Information
ChrIV:1328776 to 1330020 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2008-06-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1245 1328776..1330020 2008-06-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002840

NPL3 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NPL3]

1) Bossie MA, et al.  (1992) A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast. Mol Biol Cell 3(8):875-93
2) Kadowaki T, et al.  (1994) Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J Cell Biol 126(3):649-59
3) Ellis EM and Reid GA  (1993) The Saccharomyces cerevisiae MTS1 gene encodes a putative RNA-binding protein involved in mitochondrial protein targeting. Gene 132(2):175-83
4) Russell ID and Tollervey D  (1992) NOP3 is an essential yeast protein which is required for pre-rRNA processing. J Cell Biol 119(4):737-47
5) Wilson SM, et al.  (1994) Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol 127(5):1173-84
6) Lee MS, et al.  (1996) A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes Dev 10(10):1233-46
7) Gilbert W, et al.  (2001) Phosphorylation by Sky1p promotes Npl3p shuttling and mRNA dissociation. RNA 7(2):302-13
8) Windgassen M, et al.  (2004) Yeast shuttling SR proteins Npl3p, Gbp2p, and Hrb1p are part of the translating mRNPs, and Npl3p can function as a translational repressor. Mol Cell Biol 24(23):10479-91
9) Dermody JL, et al.  (2008) Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation. PLoS ONE 3(9):e3273
10) Kress TL, et al.  (2008) A Single SR-like Protein, Npl3, Promotes Pre-mRNA Splicing in Budding Yeast. Mol Cell 32(5):727-34