DYN2/YDR424C Summary Help

Standard Name DYN2 1
Systematic Name YDR424C
Alias SLC1 2
Feature Type ORF, Verified
Description Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments (2, 3, 4, 5, 6)
Name Description DYNein
Chromosomal Location
ChrIV:1319841 to 1319387 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All DYN2 GO evidence and references
  View Computational GO annotations for DYN2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
129 total interaction(s) for 65 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Co-crystal Structure: 5
  • Co-purification: 1
  • PCA: 1
  • Protein-peptide: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 38
  • Phenotypic Enhancement: 4
  • Positive Genetic: 7
  • Synthetic Growth Defect: 26
  • Synthetic Lethality: 23
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 92
Molecular Weight (Da) 10,441
Isoelectric Point (pI) 9.03
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1319841 to 1319387 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..25 1319841..1319817 2011-02-03 1996-07-31
Intron 26..121 1319816..1319721 2011-02-03 1996-07-31
CDS 122..144 1319720..1319698 2011-02-03 1996-07-31
Intron 145..224 1319697..1319618 2011-02-03 1996-07-31
CDS 225..455 1319617..1319387 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002832
References cited on this page View Complete Literature Guide for DYN2
1) King SM, et al.  (1996) Brain cytoplasmic and flagellar outer arm dyneins share a highly conserved Mr 8,000 light chain. J Biol Chem 271(32):19358-66
2) Dick T, et al.  (1996) Molecular and genetic characterization of SLC1, a putative Saccharomyces cerevisiae homolog of the metazoan cytoplasmic dynein light chain 1. Mol Gen Genet 251(1):38-43
3) Holleran EA, et al.  (1998) The role of the dynactin complex in intracellular motility. Int Rev Cytol 182():69-109
4) Stelter P, et al.  (2007) Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complex. Nat Cell Biol 9(7):788-96
5) Stuchell-Brereton MD, et al.  (2011) Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning. Mol Biol Cell 22(15):2690-701
6) Rao L, et al.  (2013) The yeast dynein Dyn2-Pac11 complex is a dynein dimerization/processivity factor: structural and single-molecule characterization. Mol Biol Cell 24(15):2362-2377