NCB2/YDR397C Summary

NCB2 BASIC INFORMATION

Standard Name	NCB2 1
Systematic Name	YDR397C
Alias	YDR1
Feature Type	ORF, Verified
Description	Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta (1, 2, 3)
Name Description	Negative Cofactor B 1

GO Annotations	All NCB2 GO evidence and references
	View Computational GO annotations for NCB2
Molecular Function
Manually curated	transcription coactivator activity (IDA) transcription corepressor activity (IGI, IMP)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IGI, IMP) positive regulation of transcription from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	nucleus (IPI)

Mutant Phenotype	All NCB2 Phenotype details and references
Large-scale survey
null	inviable resistance to St. John's Wort extract: decreased
overexpression	cell cycle progression in G1 phase: abnormal

Interactions	NCB2 All interactions details and references
	42 total interaction(s) for 22 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Affinity Capture-Western: 7 Biochemical Activity: 1 Co-localization: 3 PCA: 1 Reconstituted Complex: 2 Two-hybrid: 2
Genetic Interactions	Dosage Rescue: 2 Phenotypic Suppression: 1 Synthetic Growth Defect: 1 Synthetic Lethality: 1 Synthetic Rescue: 18

Sequence Information

ChrIV:1266891 to 1266359 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..37	1266891..1266855	2008-06-05	1996-07-31
Intron	38..129	1266854..1266763	2008-06-05	1996-07-31
CDS	130..533	1266762..1266359	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002805

NCB2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for NCB2

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
PDR5	Nomenclature conflict	YDR1 has been used in the literature to refer to both PDR5/YOR153W, which encodes a multi drug transporter, and NCB2/YDR397C, which encodes the beta subunit of a negative regulator of RNA Polymerase II holoenzyme.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NCB2]

1)	Gadbois EL, et al. (1997) Functional antagonism between RNA polymerase II holoenzyme and global negative regulator NC2 in vivo. Proc Natl Acad Sci U S A 94(7):3145-50
2)	Lemaire M, et al. (2000) The NC2 repressor is dispensable in yeast mutated for the Sin4p component of the holoenzyme and plays roles similar to Mot1p in vivo. Mol Microbiol 36(1):163-73
3)	Cang Y and Prelich G (2002) Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP). Proc Natl Acad Sci U S A 99(20):12727-32

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