SAC7/YDR389W Summary Help

Standard Name SAC7 1
Systematic Name YDR389W
Feature Type ORF, Verified
Description GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Suppressor of ACtin 9
Chromosomal Location
ChrIV:1252537 to 1254501 | ORF Map | GBrowse
Gbrowse
Genetic position: 220 cM
Gene Ontology Annotations All SAC7 GO evidence and references
  View Computational GO annotations for SAC7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
303 total interaction(s) for 230 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Lethality: 4
  • Dosage Rescue: 8
  • Negative Genetic: 171
  • Phenotypic Enhancement: 14
  • Phenotypic Suppression: 9
  • Positive Genetic: 24
  • Synthetic Growth Defect: 31
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 8
  • Synthetic Rescue: 18

Resources
Expression Summary
histogram
Resources
Length (a.a.) 654
Molecular Weight (Da) 73,629
Isoelectric Point (pI) 10.4
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1252537 to 1254501 | ORF Map | GBrowse
SGD ORF map
Genetic position: 220 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1965 1252537..1254501 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002797
References cited on this page View Complete Literature Guide for SAC7
1) Dunn TM and Shortle D  (1990) Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae. Mol Cell Biol 10(5):2308-14
2) Schmidt A, et al.  (1997) The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2. Cell 88(4):531-42
3) Bickle M, et al.  (1998) Cell wall integrity modulates RHO1 activity via the exchange factor ROM2. EMBO J 17(8):2235-45
4) Martin H, et al.  (2000) Regulatory mechanisms for modulation of signaling through the cell integrity Slt2-mediated pathway in Saccharomyces cerevisiae. J Biol Chem 275(2):1511-9
5) Schmidt A, et al.  (2002) The RHO1-GAPs SAC7, BEM2 and BAG7 control distinct RHO1 functions in Saccharomyces cerevisiae. Mol Microbiol 45(5):1433-41
6) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
7) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
8) Lockshon D, et al.  (2012) Rho signaling participates in membrane fluidity homeostasis. PLoS One 7(10):e45049
9) Novick P, et al.  (1989) Suppressors of yeast actin mutations. Genetics 121(4):659-74