MUS81/YDR386W Summary Help

Standard Name MUS81 1
Systematic Name YDR386W
Alias SLX3
Feature Type ORF, Verified
Description Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p (1, 2, 3, 4, 5, 6, 7)
Name Description MMS and UV Sensitive 1
Chromosomal Location
ChrIV:1246084 to 1247982 | ORF Map | GBrowse
Gene Ontology Annotations All MUS81 GO evidence and references
  View Computational GO annotations for MUS81
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
268 total interaction(s) for 133 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-Western: 5
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-purification: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 74
  • Phenotypic Enhancement: 13
  • Phenotypic Suppression: 7
  • Positive Genetic: 6
  • Synthetic Growth Defect: 68
  • Synthetic Lethality: 41
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 632
Molecular Weight (Da) 72,308
Isoelectric Point (pI) 8.61
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1246084 to 1247982 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1899 1246084..1247982 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002794
References cited on this page View Complete Literature Guide for MUS81
1) Interthal H and Heyer WD  (2000) MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Mol Gen Genet 263(5):812-27
2) Bastin-Shanower SA, et al.  (2003) The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol 23(10):3487-96
3) Fu Y and Xiao W  (2003) Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. DNA Repair (Amst) 2(12):1435-47
4) Oh SD, et al.  (2008) RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31(3):324-36
5) Jessop L and Lichten M  (2008) Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell 31(3):313-23
6) Gallo-Fernandez M, et al.  (2012) Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4. Nucleic Acids Res 40(17):8325-35
7) Pardo B and Aguilera A  (2012) Complex chromosomal rearrangements mediated by break-induced replication involve structure-selective endonucleases. PLoS Genet 8(9):e1002979