PEP7/YDR323C Summary Help

Standard Name PEP7 1
Systematic Name YDR323C
Alias VAC1 2 , VPL21 3 , VPS19 4 , 5 , VPT19 4
Feature Type ORF, Verified
Description Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance (2, 6, 7, 8, 9, 10)
Name Description carboxyPEPtidase Y-deficient 11
Chromosomal Location
ChrIV:1114027 to 1112480 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 192 cM
Gene Ontology Annotations All PEP7 GO evidence and references
  View Computational GO annotations for PEP7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
127 total interaction(s) for 105 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Biochemical Activity: 3
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 2
  • Dosage Rescue: 6
  • Negative Genetic: 45
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Positive Genetic: 13
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 13
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 515
Molecular Weight (Da) 59,443
Isoelectric Point (pI) 8.4
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1114027 to 1112480 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 192 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1548 1114027..1112480 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002731
References cited on this page View Complete Literature Guide for PEP7
1) Jones, E. and Kolodny, M.  (1985) Personal Communication, Mortimer Map Edition 9
2) Weisman LS and Wickner W  (1992) Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting. J Biol Chem 267(1):618-23
3) Raymond CK, et al.  (1992) Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants. Mol Biol Cell 3(12):1389-402
4) Robinson JS, et al.  (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
5) Rothman JH, et al.  (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
6) Webb GC, et al.  (1997) Pep7p provides a novel protein that functions in vesicle-mediated transport between the yeast Golgi and endosome. Mol Biol Cell 8(5):871-95
7) Conradt B, et al.  (1992) In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae. J Cell Biol 119(6):1469-79
8) Peterson MR, et al.  (1999) Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. Curr Biol 9(3):159-62
9) Burd CG, et al.  (1997) A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast. Mol Biol Cell 8(6):1089-104
10) Tall GG, et al.  (1999) The phosphatidylinositol 3-phosphate binding protein Vac1p interacts with a Rab GTPase and a Sec1p homologue to facilitate vesicle-mediated vacuolar protein sorting. Mol Biol Cell 10(6):1873-89
11) Jones EW  (1977) Proteinase mutants of Saccharomyces cerevisiae. Genetics 85(1):23-33