MCM21/YDR318W Summary Help

Standard Name MCM21 1
Systematic Name YDR318W
Alias CTF5 2
Feature Type ORF, Verified
Description Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 (1, 3, 4, 5)
Name Description MiniChromosome Maintenance 6
Chromosomal Location
ChrIV:1103758 to 1104947 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MCM21 GO evidence and references
  View Computational GO annotations for MCM21
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
260 total interaction(s) for 127 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-Western: 14
  • Co-crystal Structure: 1
  • Co-fractionation: 3
  • Reconstituted Complex: 3
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Lethality: 4
  • Dosage Rescue: 1
  • Negative Genetic: 118
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Positive Genetic: 5
  • Synthetic Growth Defect: 32
  • Synthetic Lethality: 31
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 368
Molecular Weight (Da) 42,971
Isoelectric Point (pI) 4.74
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1103758 to 1104947 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-27
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..52 1103758..1103809 2011-02-03 2003-09-27
Intron 53..135 1103810..1103892 2011-02-03 2003-09-27
CDS 136..1190 1103893..1104947 2011-02-03 2003-09-27
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002726
References cited on this page View Complete Literature Guide for MCM21
1) Poddar A, et al.  (1999) MCM21 and MCM22, two novel genes of the yeast Saccharomyces cerevisiae are required for chromosome transmission. Mol Microbiol 31(1):349-60
2) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
3) De Wulf P, et al.  (2003) Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes. Genes Dev 17(23):2902-21
4) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13
5) Yong-Gonzales V, et al.  (2012) The Smc5-Smc6 Complex Regulates Recombination at Centromeric Regions and Affects Kinetochore Protein Sumoylation during Normal Growth. PLoS One 7(12):e51540
6) Maine GT, et al.  (1984) Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics 106(3):365-85