GIC2/YDR309C Summary

GIC2 BASIC INFORMATION

Standard Name	GIC2 1
Systematic Name	YDR309C
Feature Type	ORF, Verified
Description	Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region (1, 2, 3, 4)
Name Description	GTPase Interactive Component 1

GO Annotations	All GIC2 GO evidence and references
	View Computational GO annotations for GIC2
Molecular Function
Manually curated	phosphatidylinositol-4,5-bisphosphate binding (IDA, IMP) Rho GTPase activator activity (IDA, IGI, IPI) Rho GTPase binding (IPI)
Biological Process
Manually curated	axial cellular bud site selection (TAS) establishment of cell polarity (IGI) regulation of exit from mitosis (IMP, IPI) Rho protein signal transduction (TAS)
Cellular Component
Manually curated	actin cap (TAS) cellular bud tip (IDA) incipient cellular bud site (IDA) mating projection tip (IDA) plasma membrane (IGI)

Mutant Phenotype	All GIC2 Phenotype details and references
Classical genetics
overexpression	budding: abnormal cell cycle progression in G2 phase: abnormal cell size: increased cellular morphology: abnormal
Large-scale survey
null	viable
overexpression	resistance to rapamycin: decreased vegetative growth: decreased

Interactions	GIC2 All interactions details and references
	78 total interaction(s) for 41 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Biochemical Activity: 2 Reconstituted Complex: 3 Two-hybrid: 26
Genetic Interactions	Dosage Lethality: 4 Dosage Rescue: 18 Phenotypic Enhancement: 4 Phenotypic Suppression: 3 Synthetic Growth Defect: 3 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 8 Synthetic Rescue: 3

Sequence Information

ChrIV:1080196 to 1079045 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1152	1080196..1079045	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002717

GIC2 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for GIC2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GIC2]

1)	Brown JL, et al. (1997) Novel Cdc42-binding proteins Gic1 and Gic2 control cell polarity in yeast. Genes Dev 11(22):2972-82
2)	Chen GC, et al. (1997) The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae. Genes Dev 11(22):2958-71
3)	Kawasaki R, et al. (2003) The upstream regulator, Rsr1p, and downstream effectors, Gic1p and Gic2p, of the Cdc42p small GTPase coordinately regulate initiation of budding in Saccharomyces cerevisiae. Genes Cells 8(3):235-50
4)	Orlando K, et al. (2008) Regulation of Gic2 Localization and Function by Phosphatidylinositol 4,5-Bisphosphate during the Establishment of Cell Polarity in Budding Yeast. J Biol Chem 283(21):14205-12

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