Standard Name	SRB7 1
Systematic Name	YDR308C
Alias	MED21 2
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p (1, 3, 4)
Name Description	Suppressor of RNA polymerase B 1

Chromosomal Location	ChrIV:1078449 to 1078027 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for SRB7
Molecular Function
Manually curated	RNA polymerase II repressing transcription factor binding (IPI) RNA polymerase II transcription coactivator activity (IDA) RNA polymerase II transcription corepressor activity (IMP, IPI)
Biological Process
Manually curated	negative regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	core mediator complex (IDA)

Classical genetics
conditional	mating efficiency: decreased utilization of carbon source: decreased
Large-scale survey
null	competitive fitness: decreased haploinsufficient inviable
reduction of function	competitive fitness: decreased resistance to methyl methanesulfonate: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	145 total interaction(s) for 52 unique genes/features.
Physical Interactions	Affinity Capture-MS: 58 Affinity Capture-RNA: 2 Affinity Capture-Western: 30 Co-crystal Structure: 2 Co-purification: 8 PCA: 2 Reconstituted Complex: 3 Two-hybrid: 24
Genetic Interactions	Dosage Rescue: 1 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Synthetic Growth Defect: 5 Synthetic Lethality: 6 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | AspGD Homologs | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:1078449 to 1078027 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..423 1078449..1078027 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002716