SRB7/YDR308C Summary

SRB7 BASIC INFORMATION

Standard Name	SRB7 1
Systematic Name	YDR308C
Alias	MED21 2
Feature Type	ORF, Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p (1, 3, 4)
Name Description	Suppressor of RNA polymerase B 1

GO Annotations	All SRB7 GO evidence and references
	View Computational GO annotations for SRB7
Molecular Function
Manually curated	RNA polymerase II transcription mediator activity (IDA)
Biological Process
Manually curated	transcription from RNA polymerase II promoter (IDA)
Cellular Component
Manually curated	mediator complex (IDA)

Mutant Phenotype	All SRB7 Phenotype details and references
Classical genetics
conditional	mating efficiency: decreased utilization of carbon source: decreased
Large-scale survey
null	competitive fitness: decreased haploinsufficient inviable

Interactions	SRB7 All interactions details and references
	122 total interaction(s) for 39 unique genes/features.
Physical Interactions	Affinity Capture-MS: 55 Affinity Capture-RNA: 1 Affinity Capture-Western: 24 Co-crystal Structure: 2 Co-purification: 8 Reconstituted Complex: 2 Two-hybrid: 22
Genetic Interactions	Phenotypic Suppression: 1 Synthetic Growth Defect: 2 Synthetic Lethality: 4 Synthetic Rescue: 1

Sequence Information

ChrIV:1078446 to 1078024 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..423	1078446..1078024	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002716

SRB7 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for SRB7

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SRB7]

1)	Hengartner CJ, et al. (1995) Association of an activator with an RNA polymerase II holoenzyme. Genes Dev 9(8):897-910
2)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3)	Gromoller A and Lehming N (2000) Srb7p is a physical and physiological target of Tup1p. EMBO J 19(24):6845-52
4)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9

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