SRB7/YDR308C Summary Help

Standard Name SRB7 1
Systematic Name YDR308C
Alias MED21 2 , SSX1 3
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p (1, 4, 5)
Name Description Suppressor of RNA polymerase B 1
Chromosomal Location
ChrIV:1078449 to 1078027 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SRB7 GO evidence and references
  View Computational GO annotations for SRB7
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
reduction of function
146 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 58
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 30
  • Co-crystal Structure: 2
  • Co-purification: 9
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 6
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 140
Molecular Weight (Da) 16,071
Isoelectric Point (pI) 4.75
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:1078449 to 1078027 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..423 1078449..1078027 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002716
References cited on this page View Complete Literature Guide for SRB7
1) Hengartner CJ, et al.  (1995) Association of an activator with an RNA polymerase II holoenzyme. Genes Dev 9(8):897-910
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Li L, et al.  (2005) Genetic interactions between mediator and the late G1-specific transcription factor Swi6 in Saccharomyces cerevisiae. Genetics 171(2):477-88
4) Gromoller A and Lehming N  (2000) Srb7p is a physical and physiological target of Tup1p. EMBO J 19(24):6845-52
5) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9