SSD1/YDR293C Summary Help

Standard Name SSD1 1
Systematic Name YDR293C
Alias CLA1 , MCS1 , RLT1 , SRK1
Feature Type ORF, Verified
Description Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function (2, 3, 4, 5, 6, 7)
Name Description Suppressor of SIT4 Deletion 1, 8
Chromosomal Location
ChrIV:1049392 to 1045640 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 176 cM
Gene Ontology Annotations All SSD1 GO evidence and references
  View Computational GO annotations for SSD1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
overexpression
reduction of function
Large-scale survey
null
overexpression
Resources
715 total interaction(s) for 472 unique genes/features.
Physical Interactions
  • Affinity Capture-Luminescence: 3
  • Affinity Capture-MS: 34
  • Affinity Capture-RNA: 8
  • Affinity Capture-Western: 13
  • Biochemical Activity: 18
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 1
  • PCA: 13
  • Reconstituted Complex: 2
  • Two-hybrid: 18

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 35
  • Negative Genetic: 411
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 8
  • Positive Genetic: 82
  • Synthetic Growth Defect: 27
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 20
  • Synthetic Rescue: 14

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,250
Molecular Weight (Da) 139,953
Isoelectric Point (pI) 7.67
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1049392 to 1045640 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 176 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3753 1049392..1045640 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002701
References cited on this page View Complete Literature Guide for SSD1
1) Sutton A, et al.  (1991) The SIT4 protein phosphatase functions in late G1 for progression into S phase. Mol Cell Biol 11(4):2133-48
2) Kaeberlein M and Guarente L  (2002) Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Genetics 160(1):83-95
3) Reinke A, et al.  (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62
4) Jansen JM, et al.  (2009) Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control. Curr Biol 19(24):2114-20
5) Ohyama Y, et al.  (2010) Saccharomyces cerevisiae Ssd1p promotes CLN2 expression by binding to the 5'-untranslated region of CLN2 mRNA. Genes Cells 15(12):1169-88
6) Kurischko C, et al.  (2011) The yeast Cbk1 kinase regulates mRNA localization via the mRNA-binding protein Ssd1. J Cell Biol 192(4):583-98
7) Kurischko C, et al.  (2011) Nucleocytoplasmic shuttling of Ssd1 defines the destiny of its bound mRNAs. Mol Microbiol 81(3):831-49
8) Stettler S, et al.  (1993) A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae. Mol Gen Genet 239(1-2):169-76