RRP45/YDR280W Summary Help

RRP45 BASIC INFORMATION

Standard Name RRP45 1
Systematic Name YDR280W
Feature Type ORF, Verified
Description Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9) (1, 2, 3, 4 and see Summary Paragraph)
Name Description Ribosomal RNA Processing 5
GO Annotations All RRP45 GO evidence and references
    View Computational GO annotations for RRP45
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All RRP45 Phenotype details and references
Classical genetics
repressible
Large-scale survey
conditional
null
Interactions RRP45 All interactions details and references
146 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 131
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Co-purification: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 3
Genetic Interactions
  • Phenotypic Enhancement: 1
  • Synthetic Lethality: 1
Sequence Information
ChrIV:1020744 to 1021661 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2008-06-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..918 1020744..1021661 2008-06-05 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002688

RRP45 RESOURCES

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Expression Summary histogram

ADDITIONAL INFORMATION for RRP45

SUMMARY PARAGRAPH for RRP45

The exosome complex possesses 3'-5' exonuclease and endoribonucleolytic activities that are essential for diverse ribonucleolytic processes in both the nucleus and the cytoplasm (6, 7, 8). The nuclear exosome is associated with the TRAMP complex and is involved in RNA catabolic processes including RNA surveillance (9, 10 and references therein), pre-mRNA turnover (11) and the production of mature 3' ends for snoRNAs, snRNAs and rRNAs (7, 12 and references therein). The cytoplasmic exosome is associated with Ski7p and the SKI complex and is involved in RNA catabolic processes that include both the routine turnover of normal mRNA (13) as well as the degradation of aberrant mRNAs (14 and references therein). The 10-subunit core exosome complex (Csl4p, Rrp4p, Rrp40p, Ski6p, Rrp42p, Rrp43p, Rrp45p, Rrp46p, Mtr3p, Dis3p) is the same in both locations, but the nuclear exosome contains an additional subunit (Rrp6p) and two additional accessory factors (Lrp1p, Mpp6p) (8).

Although the exosome was originally described as a "complex of exonucleases," with multiple subunits proposed to have RNase activity (6), later work has shown that this mechanism is unlikely in yeast. With the exception of Ski6p, none of the yeast subunits that show homology to E. coli RNase PH retain the active site residues seen in the bacterial or archael enzymes. Further research has also demonstrated that most, if not all, detectable enzymatic activity resides in the Dis3p and Rrp6p subunits (3, 4).

RRP45 encodes a core subunit of the exosome and has similarity to the RNase PH class of RNases (1, 2, and references therein). Like most exosome components, Rrp45p is highly conserved among eukaryotes, including humans (hRrp45p (PM/SCL-75, EXOSC9)) (3 and references therein). RRP45 is an essential gene (7), but cells depleted for Rrp45p have defects in pre-rRNA processing (12) and accumulate aberrant forms of rRNA (1, 12).

Last updated: 2009-09-09

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RRP45]

1) Allmang C, et al.  (1999) The yeast exosome and human PM-Scl are related complexes of 3' --> 5' exonucleases. Genes Dev 13(16):2148-58
2) Synowsky SA, et al.  (2006) Probing genuine strong interactions and post-translational modifications in the heterogeneous yeast exosome protein complex. Mol Cell Proteomics 5(9):1581-92
3) Liu Q, et al.  (2006) Reconstitution, activities, and structure of the eukaryotic RNA exosome. Cell 127(6):1223-37
4) Dziembowski A, et al.  (2007) A single subunit, Dis3, is essentially responsible for yeast exosome core activity. Nat Struct Mol Biol 14(1):15-22
5) Mitchell P, et al.  (1996) The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism. Genes Dev 10(4):502-13
6) Mitchell P, et al.  (1997) The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases. Cell 91(4):457-66
7) van Hoof A, et al.  (2000) Yeast exosome mutants accumulate 3'-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs. Mol Cell Biol 20(2):441-52
8) Synowsky SA, et al.  (2009) Comparative multiplexed mass spectrometric analyses of endogenously expressed yeast nuclear and cytoplasmic exosomes. J Mol Biol 385(4):1300-13
9) Vanacova S, et al.  (2005) A new yeast poly(A) polymerase complex involved in RNA quality control. PLoS Biol 3(6):e189
10) LaCava J, et al.  (2005) RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell 121(5):713-24
11) Bousquet-Antonelli C, et al.  (2000) Identification of a regulated pathway for nuclear pre-mRNA turnover. Cell 102(6):765-75
12) Allmang C, et al.  (2000) Degradation of ribosomal RNA precursors by the exosome. Nucleic Acids Res 28(8):1684-91
13) Anderson JS and Parker RP  (1998) The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex. EMBO J 17(5):1497-506
14) Schaeffer D, et al.  (2008) Determining in vivo activity of the yeast cytoplasmic exosome. Methods Enzymol 448:227-39