HEL2/YDR266C Summary Help

Standard Name HEL2 1
Systematic Name YDR266C
Feature Type ORF, Verified
Description RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor (1, 2, 3, 4, 5)
Name Description Histone E3 Ligase 1
Chromosomal Location
ChrIV:1002023 to 1000104 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All HEL2 GO evidence and references
  View Computational GO annotations for HEL2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 2 genes
Resources
Classical genetics
overexpression
Large-scale survey
null
overexpression
Resources
61 total interaction(s) for 47 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • Biochemical Activity: 6
  • Co-purification: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 4
  • Negative Genetic: 17
  • Phenotypic Enhancement: 6
  • Positive Genetic: 8
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 639
Molecular Weight (Da) 72,755
Isoelectric Point (pI) 9.46
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:1002023 to 1000104 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1920 1002023..1000104 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002674
References cited on this page View Complete Literature Guide for HEL2
1) Singh RK, et al.  (2012) Novel E3 Ubiquitin Ligases That Regulate Histone Protein Levels in the Budding Yeast Saccharomyces cerevisiae. PLoS One 7(5):e36295
2) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
3) Okamoto M, et al.  (2006) Glycosylphosphatidylinositol-anchored proteins are required for the transport of detergent-resistant microdomain-associated membrane proteins Tat2p and Fur4p. J Biol Chem 281(7):4013-23
4) Fleischer TC, et al.  (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307
5) Yang Y, et al.  (2010) Identifying cooperative transcription factors by combining ChIP-chip data and knockout data. Cell Res 20(11):1276-8