CHL4/YDR254W Summary

CHL4/YDR254W Summary

Standard Name	CHL4 1, 2
Systematic Name	YDR254W
Alias	CTF17 3 , MCM17 4
Feature Type	ORF, Verified
Description	Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 (5, 6, 7, 8, 9, 10)
Name Description	CHromosome Loss 5

Chromosomal Location	ChrIV:965113 to 966489 \| ORF Map \| GBrowse

Gene Ontology Annotations All CHL4 GO evidence and references

	View Computational GO annotations for CHL4
Molecular Function
Manually curated	structural molecule activity (IMP)
Biological Process
Manually curated	chromosome segregation (IMP) establishment of meiotic sister chromatid cohesion (IMP) establishment of mitotic sister chromatid cohesion (IMP) kinetochore assembly (IMP) maintenance of meiotic sister chromatid cohesion (IMP) protein localization to chromosome, centromeric region (IMP)
Cellular Component
Manually curated	condensed nuclear chromosome outer kinetochore (IDA)

Mutant phenotypes All CHL4 Phenotype evidence and references

Classical genetics
null	chromosome/plasmid maintenance: abnormal colony sectoring: increased Iml3p-YFP distribution: abnormal Ctf3p-YFP distribution: abnormal Wip1 distribution: abnormal Sgo1p distribution: abnormal resistance to benomyl: decreased resistance to bortezomib: decreased viable
unspecified	chromosome/plasmid maintenance: decreased rate chromosome/plasmid maintenance: decreased chromosome/plasmid maintenance: normal rate chromosome/plasmid maintenance: abnormal
Large-scale survey
null	competitive fitness: decreased haploinsufficient resistance to nickel sulfate: increased resistance to fluconazole: increased resistance to cycloheximide: decreased resistance to caffeine: decreased resistance to dimethyl sulfoxide: decreased resistance to BPS: decreased resistance to bleomycin: increased resistance to benzo[a]pyrene: decreased stress resistance: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All CHL4 Interaction evidence and references

	277 total interaction(s) for 138 unique genes/features.
Physical Interactions	Affinity Capture-MS: 45 Affinity Capture-RNA: 3 Affinity Capture-Western: 9 Co-crystal Structure: 1 Co-localization: 7 Two-hybrid: 6
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 4 Negative Genetic: 122 Phenotypic Enhancement: 3 Phenotypic Suppression: 3 Positive Genetic: 10 Synthetic Growth Defect: 41 Synthetic Lethality: 21 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All CHL4 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:965113 to 966489 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1377	965113..966489	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002662

References cited on this page View Complete Literature Guide for CHL4

1)	Kouprina N, et al. (1993) Identification and cloning of the CHL4 gene controlling chromosome segregation in yeast. Genetics 135(2):327-41
2)	Kouprina N, et al. (1993) Identification and genetic mapping of CHL genes controlling mitotic chromosome transmission in yeast. Yeast 9(1):11-9
3)	Spencer F, et al. (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
4)	Kroll ES, et al. (1996) Establishing genetic interactions by a synthetic dosage lethality phenotype. Genetics 143(1):95-102
5)	Kouprina NYu, et al. (1988) Genetic control of chromosome stability in the yeast Saccharomyces cerevisiae. Yeast 4(4):257-69
6)	Mythreye K and Bloom KS (2003) Differential kinetochore protein requirements for establishment versus propagation of centromere activity in Saccharomyces cerevisiae. J Cell Biol 160(6):833-43
7)	Pot I, et al. (2003) Chl4p and iml3p are two new members of the budding yeast outer kinetochore. Mol Biol Cell 14(2):460-76
8)	Marston AL, et al. (2004) A genome-wide screen identifies genes required for centromeric cohesion. Science 303(5662):1367-70
9)	Fernius J and Marston AL (2009) Establishment of cohesion at the pericentromere by the Ctf19 kinetochore subcomplex and the replication fork-associated factor, Csm3. PLoS Genet 5(9):e1000629
10)	Schleiffer A, et al. (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13