CHL4/YDR254W Summary Help

Standard Name CHL4 1, 2
Systematic Name YDR254W
Alias CTF17 3 , MCM17 4
Feature Type ORF, Verified
Description Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 (5, 6, 7, 8, 9, 10, 11, 12)
Name Description CHromosome Loss 5
Chromosomal Location
ChrIV:965113 to 966489 | ORF Map | GBrowse
Gene Ontology Annotations All CHL4 GO evidence and references
  View Computational GO annotations for CHL4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
282 total interaction(s) for 139 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 45
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 9
  • Co-crystal Structure: 2
  • Co-localization: 7
  • Reconstituted Complex: 3
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 4
  • Negative Genetic: 122
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 3
  • Positive Genetic: 10
  • Synthetic Growth Defect: 41
  • Synthetic Lethality: 21
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 458
Molecular Weight (Da) 52,671
Isoelectric Point (pI) 9.46
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:965113 to 966489 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1377 965113..966489 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002662
References cited on this page View Complete Literature Guide for CHL4
1) Kouprina N, et al.  (1993) Identification and cloning of the CHL4 gene controlling chromosome segregation in yeast. Genetics 135(2):327-41
2) Kouprina N, et al.  (1993) Identification and genetic mapping of CHL genes controlling mitotic chromosome transmission in yeast. Yeast 9(1):11-9
3) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
4) Kroll ES, et al.  (1996) Establishing genetic interactions by a synthetic dosage lethality phenotype. Genetics 143(1):95-102
5) Kouprina NYu, et al.  (1988) Genetic control of chromosome stability in the yeast Saccharomyces cerevisiae. Yeast 4(4):257-69
6) Mythreye K and Bloom KS  (2003) Differential kinetochore protein requirements for establishment versus propagation of centromere activity in Saccharomyces cerevisiae. J Cell Biol 160(6):833-43
7) Pot I, et al.  (2003) Chl4p and iml3p are two new members of the budding yeast outer kinetochore. Mol Biol Cell 14(2):460-76
8) Marston AL, et al.  (2004) A genome-wide screen identifies genes required for centromeric cohesion. Science 303(5662):1367-70
9) Fernius J and Marston AL  (2009) Establishment of cohesion at the pericentromere by the Ctf19 kinetochore subcomplex and the replication fork-associated factor, Csm3. PLoS Genet 5(9):e1000629
10) Schleiffer A, et al.  (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13
11) Matson DR, et al.  (2012) A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. Genes Dev 26(6):542-7
12) Hinshaw SM and Harrison SC  (2013) An iml3-chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation. Cell Rep 5(1):29-36