MNN10/YDR245W Summary Help

Standard Name MNN10 1, 2
Systematic Name YDR245W
Alias BED1 3 , SLC2 4 , 5 , REC41 6
Feature Type ORF, Verified
Description Subunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p (2, 7)
Name Description MaNNosyltransferase
Chromosomal Location
ChrIV:952800 to 953981 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MNN10 GO evidence and references
  View Computational GO annotations for MNN10
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
null
overexpression
Resources
481 total interaction(s) for 359 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 29
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Co-purification: 1
  • PCA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 313
  • Phenotypic Enhancement: 2
  • Positive Genetic: 45
  • Synthetic Growth Defect: 52
  • Synthetic Lethality: 28

Resources
Expression Summary
histogram
Resources
Length (a.a.) 393
Molecular Weight (Da) 46,748
Isoelectric Point (pI) 6.87
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:952800 to 953981 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1182 952800..953981 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002653
References cited on this page View Complete Literature Guide for MNN10
1) Ballou L, et al.  (1989) Protein glycosylation defects in the Saccharomyces cerevisiae mnn7 mutant class. Support for the stop signal proposed for regulation of outer chain elongation. J Biol Chem 264(20):11857-64
2) Dean N and Poster JB  (1996) Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases. Glycobiology 6(1):73-81
3) Mondesert G and Reed SI  (1996) BED1, a gene encoding a galactosyltransferase homologue, is required for polarized growth and efficient bud emergence in Saccharomyces cerevisiae. J Cell Biol 132(1-2):137-51
4) Karpova TS, et al.  (1993) Mutations that enhance the cap2 null mutant phenotype in Saccharomyces cerevisiae affect the actin cytoskeleton, morphogenesis and pattern of growth. Genetics 135(3):693-709
5) Karpova TS, et al.  (1995) Actin filaments in yeast are unstable in the absence of capping protein or fimbrin. J Cell Biol 131(6 Pt 1):1483-93
6) Chepurnaya OV, et al.  (2001) The REC41 gene of Saccharomyces cerevisiae: isolation and genetic analysis. Mutat Res 486(1):41-52
7) Jungmann J, et al.  (1999) The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi mannosyltransferase complex. J Biol Chem 274(10):6579-85