LYS4/YDR234W Summary Help

Standard Name LYS4 1
Systematic Name YDR234W
Alias LYS3
Feature Type ORF, Verified
Description Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway (2 and see Summary Paragraph)
Name Description LYSine requiring 1
Chromosomal Location
ChrIV:931129 to 933210 | ORF Map | GBrowse
Genetic position: 145.87 cM
Gene Ontology Annotations All LYS4 GO evidence and references
  View Computational GO annotations for LYS4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 6 genes
Classical genetics
Large-scale survey
25 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Protein-peptide: 1

Genetic Interactions
  • Synthetic Lethality: 12
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 693
Molecular Weight (Da) 75,150
Isoelectric Point (pI) 7.36
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:931129 to 933210 | ORF Map | GBrowse
Genetic position: 145.87 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2082 931129..933210 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002642

About lysine biosynthesis

S. cerevisiae synthesizes the essential amino acid L-lysine via the L-alpha-aminoadipic acid pathway instead of the diaminopmelate pathway (3). Originally proposed to be characteristic of fungi, recent studies suggest prokaryotes also synthesize lysine via the alpha-aminoadipic acid pathway (4). Intermediates in this pathway are often incorporated into secondary metabolites. For example, it has been well- studied that alpha-aminoadipate is required for penicillin production (3). Regulation of the lysine biosynthetic pathway in S. cerevisiae is an interaction between general amino acid control (via Gcn4p) (5), feedback inhibition of homocitrate synthase activity by lysine (6), and induction of Lys14p by alpha-aminoadipate semialdehyde (7).

Last updated: 2007-10-04 Contact SGD

References cited on this page View Complete Literature Guide for LYS4
1) Wang L, et al.  (1989) Cloning and physical characterization of linked lysine genes (lys4, lys15) of Saccharomyces cerevisiae. Curr Genet 16(1):7-12
2) Maragoudakis ME and Strassman M  (1966) Homocitric acid accumulation by a lysine-requiring yeast mutant. J Biol Chem 241(3):695-9
3) Zabriskie TM and Jackson MD  (2000) Lysine biosynthesis and metabolism in fungi. Nat Prod Rep 17(1):85-97
4) Nishida H and Nishiyama M  (2000) What is characteristic of fungal lysine synthesis through the alpha-aminoadipate pathway? J Mol Evol 51(3):299-302
5) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Feller A, et al.  (1999) In Saccharomyces cerevisae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p. Eur J Biochem 261(1):163-70
7) El Alami M, et al.  (2000) Characterisation of a tripartite nuclear localisation sequence in the regulatory protein Lys14 of Saccharomyces cerevisiae. Curr Genet 38(2):78-86