RTN1/YDR233C Summary

RTN1/YDR233C Summary

Standard Name	RTN1 1
Systematic Name	YDR233C
Feature Type	ORF, Verified
Description	Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
Name Description	ReTiculoN-like 1

Chromosomal Location	ChrIV:930357 to 929470 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All RTN1 GO evidence and references

	View Computational GO annotations for RTN1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	endoplasmic reticulum organization (IGI) endoplasmic reticulum tubular network maintenance (IGI) endoplasmic reticulum tubular network organization (IGI, IMP, IPI) nuclear pore complex assembly (IGI)
Cellular Component
Manually curated	cortical endoplasmic reticulum (IDA) endoplasmic reticulum (IDA) endoplasmic reticulum tubular network (IDA) Golgi apparatus (IDA) integral to endoplasmic reticulum membrane (IDA)
High-throughput	endoplasmic reticulum (IDA) mitochondrion (IDA)

Mutant phenotypes All RTN1 Phenotype evidence and references

Classical genetics
null	tellurium atom accumulation: increased resistance to tellurite: decreased viable
Large-scale survey
null	competitive fitness: decreased endocytosis: decreased Fus-Mid-GFP distribution: abnormal replicative lifespan: increased resistance to hygromycin B: decreased viable
overexpression	inviable vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All RTN1 Interaction evidence and references

	158 total interaction(s) for 111 unique genes/features.
Physical Interactions	Affinity Capture-MS: 31 Affinity Capture-RNA: 4 Affinity Capture-Western: 14 Co-purification: 1 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 6 Negative Genetic: 46 Phenotypic Enhancement: 10 Phenotypic Suppression: 8 Positive Genetic: 15 Synthetic Growth Defect: 9 Synthetic Lethality: 11 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Mass Spec

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All RTN1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:930357 to 929470 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..888	930357..929470	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002641

References cited on this page View Complete Literature Guide for RTN1

1)	Oertle T, et al. (2003) A reticular rhapsody: phylogenic evolution and nomenclature of the RTN/Nogo gene family. FASEB J 17(10):1238-47
2)	Geng J, et al. (2005) Saccharomyces cerevisiae Rab-GDI displacement factor ortholog Yip3p forms distinct complexes with the Ypt1 Rab GTPase and the reticulon Rtn1p. Eukaryot Cell 4(7):1166-74
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Voeltz GK, et al. (2006) A class of membrane proteins shaping the tubular endoplasmic reticulum. Cell 124(3):573-86
5)	De Craene JO, et al. (2006) Rtn1p is involved in structuring the cortical endoplasmic reticulum. Mol Biol Cell 17(7):3009-20
6)	Feng D, et al. (2007) The transmembrane domain is sufficient for Sbh1p function, its association with the Sec61 complex, and interaction with Rtn1p. J Biol Chem 282(42):30618-28
7)	Hu J, et al. (2008) Membrane proteins of the endoplasmic reticulum induce high-curvature tubules. Science 319(5867):1247-50
8)	Shibata Y, et al. (2008) The reticulon and dp1/yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum. J Biol Chem 283(27):18892-904
9)	Dawson TR, et al. (2009) ER membrane-bending proteins are necessary for de novo nuclear pore formation. J Cell Biol 184(5):659-75
10)	Hu J, et al. (2009) A class of dynamin-like GTPases involved in the generation of the tubular ER network. Cell 138(3):549-61
11)	West M, et al. (2011) A 3D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature. J Cell Biol 193(2):333-46
12)	Voss C, et al. (2012) ER-shaping proteins facilitate lipid exchange between the ER and mitochondria in S. cerevisiae. J Cell Sci 125(Pt 20):4791-9