PCF11/YDR228C Summary Help

Standard Name PCF11 1
Systematic Name YDR228C
Feature Type ORF, Verified
Description mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5, 6, 7)
Name Description Protein 1 of Cleavage and polyadenylation Factor I 1
Chromosomal Location
ChrIV:923806 to 921926 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PCF11 GO evidence and references
  View Computational GO annotations for PCF11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
reduction of function
155 total interaction(s) for 85 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 36
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 19
  • Co-crystal Structure: 1
  • Co-purification: 1
  • Protein-peptide: 3
  • Protein-RNA: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 19

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 45
  • Positive Genetic: 6
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 7
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 626
Molecular Weight (Da) 71,898
Isoelectric Point (pI) 7.81
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:923806 to 921926 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1881 923806..921926 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002636
References cited on this page View Complete Literature Guide for PCF11
1) Amrani N, et al.  (1997) PCF11 encodes a third protein component of yeast cleavage and polyadenylation factor I. Mol Cell Biol 17(3):1102-9
2) Birse CE, et al.  (1998) Coupling termination of transcription to messenger RNA maturation in yeast. Science 280(5361):298-301
3) Barilla D, et al.  (2001) Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 98(2):445-50
4) de Vries H, et al.  (2000) Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors. EMBO J 19(21):5895-904
5) Sadowski M, et al.  (2003) Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination. EMBO J 22(9):2167-77
6) Medler S, et al.  (2011) Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping. J Biol Chem 286(39):33709-18
7) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30