UPC2/YDR213W Summary

UPC2/YDR213W Summary

Standard Name	UPC2 1
Systematic Name	YDR213W
Alias	MOX4 2
Feature Type	ORF, Verified
Description	Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description	UPtake Control 1, 3

Chromosomal Location	ChrIV:889751 to 892492 \| ORF Map \| GBrowse

Gene Ontology Annotations All UPC2 GO evidence and references

	View Computational GO annotations for UPC2
Molecular Function
Manually curated	RNA polymerase II core promoter proximal region sequence-specific DNA binding (IDA) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (IDA, IMP)
Biological Process
Manually curated	cellular response to hypoxia (IMP) positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of sterol import by positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	membrane (IDA) nucleus (IC) perinuclear region of cytoplasm (IDA)
High-throughput	cytoplasm (IDA)

Regulatory Role


Resources	ScerTF \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All UPC2 Phenotype evidence and references

Classical genetics
null	ergosterol accumulation: decreased cholesterol accumulation: decreased resistance to fluconazole: decreased viable
overexpression	budding index: abnormal cell cycle progression in G1 phase: abnormal cell size: increased cellular morphology: abnormal
unspecified	sterol accumulation: decreased
Large-scale survey
null	competitive fitness: decreased haploproficient resistance to ketoconazole: decreased resistance to 4-nitroquinoline N-oxide: normal resistance to oligomycin: normal resistance to chloramphenicol: normal resistance to cycloheximide: normal resistance to rhodamine 6-G: normal vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All UPC2 Interaction evidence and references

	108 total interaction(s) for 91 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 1 Two-hybrid: 2
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Negative Genetic: 52 Phenotypic Enhancement: 2 Phenotypic Suppression: 14 Positive Genetic: 14 Synthetic Growth Defect: 2 Synthetic Lethality: 10 Synthetic Rescue: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All UPC2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:889751 to 892492 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2742	889751..892492	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002621

References cited on this page View Complete Literature Guide for UPC2

1)	Lewis TL, et al. (1988) Pleiotropic mutations in Saccharomyces cerevisiae affecting sterol uptake and metabolism. Yeast 4(2):93-106
2)	Abramova NE, et al. (2001) Regulatory mechanisms controlling expression of the DAN/TIR mannoprotein genes during anaerobic remodeling of the cell wall in Saccharomyces cerevisiae. Genetics 157(3):1169-77
3)	Crowley JH, et al. (1998) A mutation in a purported regulatory gene affects control of sterol uptake in Saccharomyces cerevisiae. J Bacteriol 180(16):4177-83
4)	Shianna KV, et al. (2001) Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake. J Bacteriol 183(3):830-4
5)	Abramova N, et al. (2001) Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae. J Bacteriol 183(9):2881-7
6)	Vik A and Rine J (2001) Upc2p and Ecm22p, dual regulators of sterol biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol 21(19):6395-405
7)	Kwast KE, et al. (2002) Genomic analyses of anaerobically induced genes in Saccharomyces cerevisiae: functional roles of Rox1 and other factors in mediating the anoxic response. J Bacteriol 184(1):250-65
8)	Wilcox LJ, et al. (2002) Transcriptional profiling identifies two members of the ATP-binding cassette transporter superfamily required for sterol uptake in yeast. J Biol Chem 277(36):32466-72
9)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
10)	Marie C, et al. (2008) Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae. Fungal Genet Biol 45(10):1430-8