UME6/YDR207C Summary Help

UME6 BASIC INFORMATION

Standard Name UME6 1
Systematic Name YDR207C
Alias CAR80 1 , 2 , NIM2 , RIM16 3
Feature Type ORF, Verified
Description Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p (2, 4, 5, 6, 7, 8, 9, 10 and see Summary Paragraph)
Name Description Unscheduled Meiotic gene Expression 11
GO Annotations All UME6 GO evidence and references
    View Computational GO annotations for UME6
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulatory Role
Binding motifs UME6 Transcription Factor Binding Sites and References
  • TAGCCGCCGA
  • TAGCCGCCSA
Mutant Phenotype All UME6 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions UME6 All interactions details and references
197 total interaction(s) for 156 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Biochemical Activity: 5
  • Reconstituted Complex: 1
  • Two-hybrid: 14
Genetic Interactions
  • Phenotypic Enhancement: 91
  • Phenotypic Suppression: 24
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 19
  • Synthetic Rescue: 2
Sequence Information
ChrIV:867520 to 865010 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2008-06-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2511 867520..865010 2008-06-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002615

UME6 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for UME6

SUMMARY PARAGRAPH for UME6

UME6 ("Unscheduled Meiotic gene Expression") is a key transcriptional regulator of early meiotic genes, such as SPO11, SPO13, and IME2 (12). UME6 is required for their repression during mitosis and for their induction during meiosis (2, 6). This dual role is achieved by the interaction of Ume6p with different regulatory proteins during mitosis and meiosis (13, 14). Ume6p provides target specificity by binding to the URS1 sequence element (TAGCCGCCGA) that is located upstream from many early meiosis-specific genes (15, 2). During mitosis, Ume6p recruits Sin3p and Rpd3p to repress transcription by hypoacetylation of histone H3 and histone H4 (14, 16, 17). Upon entry into meiosis, Ume6p becomes hyper- phosphorylated by Rim11p and Mck1p. When phosphorylated, Ume6p interacts with the meiosis-specific transcriptional activator Ime1p (13, 7, 18).

In addition to the regulation of meiosis-specific genes, UME6 has been implicated in the transcriptional regulation of genes involved in arginine catabolism (CAR1 and CAR2) (2), peroxisomal function (FOX3) (19), and DNA repair genes (PHR1) (20). Like the early meiosis- specific genes, these genes contain URS1 sequence elements.

Ume6p is a zinc cluster DNA binding protein that contains six conserved cysteines (2). It binds 2 Zn(II) ions to form a binuclear zinc cluster (4). It, however, does not contain an activation domain (21).

Last updated: 2002-04-01

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for UME6]

1) Park HD, et al.  (1992) The yeast UME6 gene product is required for transcriptional repression mediated by the CAR1 URS1 repressor binding site. Nucleic Acids Res 20(8):1909-15
2) Strich R, et al.  (1994) UME6 is a key regulator of nitrogen repression and meiotic development. Genes Dev 8(7):796-810
3) Mitchell AP and Bowdish KS  (1992) Selection for early meiotic mutants in yeast. Genetics 131(1):65-72
4) Anderson SF, et al.  (1995) UME6, a negative regulator of meiosis in Saccharomyces cerevisiae, contains a C-terminal Zn2Cys6 binuclear cluster that binds the URS1 DNA sequence in a zinc-dependent manner. Protein Sci 4(9):1832-43
5) Bowdish KS and Mitchell AP  (1993) Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae. Mol Cell Biol 13(4):2172-81
6) Steber CM and Esposito RE  (1995) UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression. Proc Natl Acad Sci U S A 92(26):12490-4
7) Malathi K, et al.  (1997) Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p. Mol Cell Biol 17(12):7230-6
8) Messenguy F, et al.  (2000) In Saccharomyces cerevisiae, expression of arginine catabolic genes CAR1 and CAR2 in response to exogenous nitrogen availability is mediated by the Ume6 (CargRI)-Sin3 (CargRII)-Rpd3 (CargRIII) complex. J Bacteriol 182(11):3158-64
9) Elkhaimi M, et al.  (2000) Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes. Nucleic Acids Res 28(16):3160-7
10) Williams RM, et al.  (2002) The Ume6 regulon coordinates metabolic and meiotic gene expression in yeast. Proc Natl Acad Sci U S A 99(21):13431-6
11) Strich R, et al.  (1989) Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast. Proc Natl Acad Sci U S A 86(24):10018-22
12) Vershon AK and Pierce M  (2000) Transcriptional regulation of meiosis in yeast. Curr Opin Cell Biol 12(3):334-9
13) Rubin-Bejerano I, et al.  (1996) Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1. Mol Cell Biol 16(5):2518-26
14) Kadosh D and Struhl K  (1997) Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters. Cell 89(3):365-71
15) Buckingham LE, et al.  (1990) Nucleotide sequence and promoter analysis of SPO13, a meiosis-specific gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 87(23):9406-10
16) Rundlett SE, et al.  (1998) Transcriptional repression by UME6 involves deacetylation of lysine 5 of histone H4 by RPD3. Nature 392(6678):831-5
17) Kadosh D and Struhl K  (1998) Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo. Mol Cell Biol 18(9):5121-7
18) Xiao Y and Mitchell AP  (2000) Shared roles of yeast glycogen synthase kinase 3 family members in nitrogen-responsive phosphorylation of meiotic regulator Ume6p. Mol Cell Biol 20(15):5447-53
19) Einerhand AW, et al.  (1995) The upstream region of the FOX3 gene encoding peroxisomal 3-oxoacyl-coenzyme A thiolase in Saccharomyces cerevisiae contains ABF1- and replication protein A-binding sites that participate in its regulation by glucose repression. Mol Cell Biol 15(6):3405-14
20) Sweet DH, et al.  (1997) Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae. Mol Cell Biol 17(11):6223-35
21) Washburn BK and Esposito RE  (2001) Identification of the Sin3-binding site in Ume6 defines a two-step process for conversion of Ume6 from a transcriptional repressor to an activator in yeast. Mol Cell Biol 21(6):2057-69