UME6/YDR207C Summary Help

Standard Name UME6 1
Systematic Name YDR207C
Alias CAR80 1 , 2 , NIM2 , RIM16 3
Feature Type ORF, Verified
Description Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p (2, 4, 5, 6, 7, 8, 9, 10, 11, 12 and see Summary Paragraph)
Name Description Unscheduled Meiotic gene Expression 13
Chromosomal Location
ChrIV:867522 to 865012 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All UME6 GO evidence and references
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 580 genes
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
532 total interaction(s) for 363 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 35
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Biochemical Activity: 7
  • Reconstituted Complex: 1
  • Two-hybrid: 15

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 325
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Positive Genetic: 94
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 19
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 836
Molecular Weight (Da) 91,123
Isoelectric Point (pI) 10.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:867522 to 865012 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2511 867522..865012 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002615
SUMMARY PARAGRAPH for UME6

UME6 (Unscheduled Meiotic gene Expression) is a key transcriptional regulator of early meiotic genes, such as SPO11, SPO13, and IME2 (14). UME6 is required for their repression during mitosis and its destruction during meiosis is required for their meiotic induction (2, 15). This C6 zinc cluster DNA binding protein provides target specificity by binding to the URS1 sequence element (TAGCCGCCGA) that is located upstream of many early meiosis-specific genes (16, 2). During mitosis, Ume6p recruits Sin3p and Rpd3p, subunits of a histone deacetylase complex, resulting in transcriptional repression of target genes by hypoacetylation of histone H3 and histone H4 (17, 18, 19). Upon entry into meiosis, Ume6p becomes hyperphosphorylated by Rim11p and Mck1p. When phosphorylated, Ume6p interacts with the meiosis-specific transcriptional activator Ime1p (20, 7, 21). This association triggers Ume6p destruction by the APC/C-Cdc20 ubiquitin ligase, permitting early meiotic gene expression (15).

In addition to the regulation of meiosis-specific genes, UME6 has been implicated in the transcriptional regulation of genes involved in arginine catabolism (CAR1 and CAR2) (2), peroxisomal function (FOX3) (22), and DNA repair genes (PHR1) (23). Like the early meiosis- specific genes, these genes contain URS1 sequence elements.

Ume6p is a zinc cluster DNA binding protein that contains six conserved cysteines (2). It binds 2 Zn(II) ions to form a binuclear zinc cluster (4). It, however, does not contain an activation domain (24).

Last updated: 2002-04-01 Contact SGD

References cited on this page View Complete Literature Guide for UME6
1) Park HD, et al.  (1992) The yeast UME6 gene product is required for transcriptional repression mediated by the CAR1 URS1 repressor binding site. Nucleic Acids Res 20(8):1909-15
2) Strich R, et al.  (1994) UME6 is a key regulator of nitrogen repression and meiotic development. Genes Dev 8(7):796-810
3) Mitchell AP and Bowdish KS  (1992) Selection for early meiotic mutants in yeast. Genetics 131(1):65-72
4) Anderson SF, et al.  (1995) UME6, a negative regulator of meiosis in Saccharomyces cerevisiae, contains a C-terminal Zn2Cys6 binuclear cluster that binds the URS1 DNA sequence in a zinc-dependent manner. Protein Sci 4(9):1832-43
5) Bowdish KS and Mitchell AP  (1993) Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae. Mol Cell Biol 13(4):2172-81
6) Steber CM and Esposito RE  (1995) UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression. Proc Natl Acad Sci U S A 92(26):12490-4
7) Malathi K, et al.  (1997) Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p. Mol Cell Biol 17(12):7230-6
8) Messenguy F, et al.  (2000) In Saccharomyces cerevisiae, expression of arginine catabolic genes CAR1 and CAR2 in response to exogenous nitrogen availability is mediated by the Ume6 (CargRI)-Sin3 (CargRII)-Rpd3 (CargRIII) complex. J Bacteriol 182(11):3158-64
9) Elkhaimi M, et al.  (2000) Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes. Nucleic Acids Res 28(16):3160-7
10) Williams RM, et al.  (2002) The Ume6 regulon coordinates metabolic and meiotic gene expression in yeast. Proc Natl Acad Sci U S A 99(21):13431-6
11) Carrozza MJ, et al.  (2005) Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex. Biochim Biophys Acta 1731(2):77-87; discussion 75-6
12) Yadon AN, et al.  (2013) DNA looping facilitates targeting of a chromatin remodeling enzyme. Mol Cell 50(1):93-103
13) Strich R, et al.  (1989) Identification of negative regulatory genes that govern the expression of early meiotic genes in yeast. Proc Natl Acad Sci U S A 86(24):10018-22
14) Vershon AK and Pierce M  (2000) Transcriptional regulation of meiosis in yeast. Curr Opin Cell Biol 12(3):334-9
15) Mallory MJ, et al.  (2007) Meiosis-specific destruction of the Ume6p repressor by the Cdc20-directed APC/C. Mol Cell 27(6):951-61
16) Buckingham LE, et al.  (1990) Nucleotide sequence and promoter analysis of SPO13, a meiosis-specific gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 87(23):9406-10
17) Kadosh D and Struhl K  (1997) Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters. Cell 89(3):365-71
18) Rundlett SE, et al.  (1998) Transcriptional repression by UME6 involves deacetylation of lysine 5 of histone H4 by RPD3. Nature 392(6678):831-5
19) Kadosh D and Struhl K  (1998) Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo. Mol Cell Biol 18(9):5121-7
20) Rubin-Bejerano I, et al.  (1996) Induction of meiosis in Saccharomyces cerevisiae depends on conversion of the transcriptional represssor Ume6 to a positive regulator by its regulated association with the transcriptional activator Ime1. Mol Cell Biol 16(5):2518-26
21) Xiao Y and Mitchell AP  (2000) Shared roles of yeast glycogen synthase kinase 3 family members in nitrogen-responsive phosphorylation of meiotic regulator Ume6p. Mol Cell Biol 20(15):5447-53
22) Einerhand AW, et al.  (1995) The upstream region of the FOX3 gene encoding peroxisomal 3-oxoacyl-coenzyme A thiolase in Saccharomyces cerevisiae contains ABF1- and replication protein A-binding sites that participate in its regulation by glucose repression. Mol Cell Biol 15(6):3405-14
23) Sweet DH, et al.  (1997) Role of UME6 in transcriptional regulation of a DNA repair gene in Saccharomyces cerevisiae. Mol Cell Biol 17(11):6223-35
24) Washburn BK and Esposito RE  (2001) Identification of the Sin3-binding site in Ume6 defines a two-step process for conversion of Ume6 from a transcriptional repressor to an activator in yeast. Mol Cell Biol 21(6):2057-69
25) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
26) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87
27) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104