CAB5/YDR196C Summary Help

Standard Name CAB5 1
Systematic Name YDR196C
Feature Type ORF, Verified
Description Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets (1, 2, 3, 4, 5, 6, 7)
Chromosomal Location
ChrIV:850999 to 850274 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CAB5 GO evidence and references
  View Computational GO annotations for CAB5
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Pathways
Classical genetics
conditional
null
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
42 total interaction(s) for 24 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 4

Genetic Interactions
  • Negative Genetic: 23
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 241
Molecular Weight (Da) 27,340
Isoelectric Point (pI) 8.83
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:850999 to 850274 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..726 850999..850274 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002604
References cited on this page View Complete Literature Guide for CAB5
1) Olzhausen J, et al.  (2009) Genetic analysis of coenzyme A biosynthesis in the yeast Saccharomyces cerevisiae: identification of a conditional mutation in the pantothenate kinase gene CAB1. Curr Genet 55(2):163-73
2) Giaever G, et al.  (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
3) Tatusov RL, et al.  (2000) The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res 28(1):33-6
4) Daugherty M, et al.  (2002) Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. J Biol Chem 277(24):21431-9
5) Reinders J, et al.  (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54
6) Olzhausen J, et al.  (2013) Molecular characterization of the heteromeric coenzyme A-synthesizing protein complex (CoA-SPC) in the yeast Saccharomyces cerevisiae. FEMS Yeast Res 13(6):565-73
7) Currie E, et al.  (2014) High-Confidence Proteomic Analysis of Yeast Lipid Droplets Identifies Additional Droplet Proteins and Reveals Connections to Dolichol Synthesis and Sterol Acetylation. J Lipid Res ()