RVB1/YDR190C Summary Help

Standard Name RVB1 1
Systematic Name YDR190C
Alias TIP49A 2 , TIH1 3 , TIP49 4
Feature Type ORF, Verified
Description ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly (1, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
Name Description RuVB-like 6
Chromosomal Location
ChrIV:841995 to 840604 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All RVB1 GO evidence and references
  View Computational GO annotations for RVB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
reduction of function
277 total interaction(s) for 156 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 150
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 17
  • Biochemical Activity: 2
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-purification: 4
  • PCA: 2
  • Reconstituted Complex: 10
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 50
  • Positive Genetic: 16
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 1

Expression Summary
Length (a.a.) 463
Molecular Weight (Da) 50,453
Isoelectric Point (pI) 5.7
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:841995 to 840604 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1392 841995..840604 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002598
References cited on this page View Complete Literature Guide for RVB1
1) Shen X, et al.  (2000) A chromatin remodelling complex involved in transcription and DNA processing. Nature 406(6795):541-4
2) Kanemaki M, et al.  (1999) TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a. J Biol Chem 274(32):22437-44
3) Lim CR, et al.  (2000) The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription. J Biol Chem 275(29):22409-17
4) Wood MA, et al.  (2000) An ATPase/helicase complex is an essential cofactor for oncogenic transformation by c-Myc. Mol Cell 5(2):321-30
5) Qiu XB, et al.  (1998) An eukaryotic RuvB-like protein (RUVBL1) essential for growth. J Biol Chem 273(43):27786-93
6) Jonsson ZO, et al.  (2001) Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes. J Biol Chem 276(19):16279-88
7) King TH, et al.  (2001) A well-connected and conserved nucleoplasmic helicase is required for production of box C/D and H/ACA snoRNAs and localization of snoRNP proteins. Mol Cell Biol 21(22):7731-46
8) Krogan NJ, et al.  (2003) A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1. Mol Cell 12(6):1565-76
9) Mizuguchi G, et al.  (2004) ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex. Science 303(5656):343-8
10) Jonsson ZO, et al.  (2004) Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex. Mol Cell 16(3):465-77
11) Zhao R, et al.  (2005) Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone. Cell 120(5):715-27
12) Gribun A, et al.  (2008) Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex. J Mol Biol 376(5):1320-33
13) Torreira E, et al.  (2008) Architecture of the pontin/reptin complex, essential in the assembly of several macromolecular complexes. Structure 16(10):1511-20
14) Zhao R, et al.  (2008) Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation. J Cell Biol 180(3):563-78
15) Kakihara Y and Houry WA  (2012) The R2TP complex: discovery and functions. Biochim Biophys Acta 1823(1):101-7