SLY1/YDR189W Summary

SLY1 BASIC INFORMATION

Standard Name	SLY1
Systematic Name	YDR189W
Feature Type	ORF, Verified
Description	Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex (1, 2)
Name Description	Suppressor of Loss of Ypt1

GO Annotations	All SLY1 GO evidence and references
	View Computational GO annotations for SLY1
Molecular Function
Manually curated	SNARE binding (IDA)
Biological Process
Manually curated	ER to Golgi vesicle-mediated transport (IMP) protein retention in Golgi apparatus (IGI, IMP) retrograde vesicle-mediated transport, Golgi to ER (IMP)
Cellular Component
Manually curated	endoplasmic reticulum (IDA) ER to Golgi transport vesicle (IDA) Golgi apparatus (IDA)

Mutant Phenotype	All SLY1 Phenotype details and references
Classical genetics
conditional	heat sensitivity: increased Kar2p, Rer1p or Sed5p transport: abnormal
Large-scale survey
null	inviable
overexpression	vegetative growth: decreased
repressible	mitochondrial morphology: abnormal

Interactions	SLY1 All interactions details and references
	112 total interaction(s) for 49 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-Western: 23 Biochemical Activity: 1 Co-crystal Structure: 1 PCA: 7 Reconstituted Complex: 14 Two-hybrid: 6
Genetic Interactions	Dosage Rescue: 25 Phenotypic Suppression: 8 Synthetic Lethality: 13 Synthetic Rescue: 8

Sequence Information

ChrIV:838390 to 840390 | |

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2001	838390..840390	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002597

SLY1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for SLY1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SLY1]

1)	Cao X, et al. (1998) Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J 17(8):2156-65
2)	Kosodo Y, et al. (2003) Cooperation of Sly1/SM-family protein and sec18/NSF of Saccharomyces cerevisiae in disassembly of cis-SNARE membrane-protein complexes. Biosci Biotechnol Biochem 67(2):448-50

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