TRM82/YDR165W Summary Help

Standard Name TRM82 1
Systematic Name YDR165W
Feature Type ORF, Verified
Description Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5)
Name Description Transfer RNA Methyltransferase 1
Chromosomal Location
ChrIV:784871 to 786205 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRM82 GO evidence and references
  View Computational GO annotations for TRM82
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
49 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • Co-crystal Structure: 1
  • Co-purification: 1
  • PCA: 2
  • Protein-peptide: 1
  • Reconstituted Complex: 2

Genetic Interactions
  • Negative Genetic: 21
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 8
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 444
Molecular Weight (Da) 50,488
Isoelectric Point (pI) 6.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:784871 to 786205 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1335 784871..786205 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002572
References cited on this page View Complete Literature Guide for TRM82
1) Alexandrov A, et al.  (2002) Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA 8(10):1253-66
2) Matsumoto K, et al.  (2007) RNA recognition mechanism of eukaryote tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex). FEBS Lett 581(8):1599-604
3) Matsumoto K, et al.  (2008) Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system. J Biotechnol 133(4):453-60
4) Leulliot N, et al.  (2008) Structure of the Yeast tRNA m7G Methylation Complex. Structure 16(1):52-61
5) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30