Standard Name	TRM82 1
Systematic Name	YDR165W
Feature Type	ORF, Verified
Description	Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia (1, 2, 3, 4, 5)
Name Description	Transfer RNA Methyltransferase 1

Chromosomal Location	ChrIV:784871 to 786205 | ORF Map | GBrowse

	View Computational GO annotations for TRM82
Molecular Function
Manually curated	contributes_to tRNA (guanine-N7-)-methyltransferase activity (IDA, IMP)
Biological Process
Manually curated	tRNA methylation (IDA, IMP)
Cellular Component
Manually curated	cytosol (IDA) nucleus (IDA) tRNA methyltransferase complex (IDA)
High-throughput	nucleus (IDA)

Classical genetics
null	resistance to 5-fluorouracil: decreased
Large-scale survey
null	competitive fitness: decreased haploproficient resistance to 5-fluorouracil: decreased starvation resistance: decreased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	47 total interaction(s) for 31 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2 Affinity Capture-Western: 1 Co-crystal Structure: 1 Co-purification: 1 PCA: 2 Protein-peptide: 1 Reconstituted Complex: 2
Genetic Interactions	Negative Genetic: 21 Phenotypic Enhancement: 1 Synthetic Growth Defect: 8 Synthetic Lethality: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:784871 to 786205 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1335 784871..786205 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002572