Standard Name	NBP2
Systematic Name	YDR162C
Feature Type	ORF, Verified
Description	Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) (1, 2, 3)
Name Description	Nap1 Binding Protein 4

Chromosomal Location	ChrIV:781100 to 780390 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for NBP2
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	hyperosmotic response (IMP, IPI) inactivation of MAPK activity involved in cell wall biogenesis (IMP, IPI) negative regulation of protein kinase activity (IDA) response to heat (IMP)
Cellular Component
Manually curated	cytoplasm (IDA)
High-throughput	nucleus (IDA)

Classical genetics
null	metal resistance: decreased resistance to methotrexate: increased resistance to 2'-amino-3'-methylflavone: increased resistance to 4-aminofolic acid: increased
Large-scale survey
null	auxotrophy chitin deposition: increased competitive fitness: decreased haploproficient heat sensitivity: increased hyperosmotic stress resistance: decreased resistance to doxorubicin: decreased resistance to amiodarone: decreased resistance to oleate: decreased resistance to Calcofluor White: decreased resistance to wortmannin: increased resistance to cycloheximide: decreased resistance to streptomycin: decreased resistance to hydroxyurea: decreased resistance to ethanol: decreased resistance to 5-fluorouracil: decreased resistance to propan-1-ol: decreased resistance to nickel sulfate: decreased resistance to CTBT: decreased resistance to formaldehyde: decreased resistance to arsenite(3-): decreased resistance to hydrogen chloride: decreased resistance to neothyonidioside: decreased resistance to sirolimus: decreased resistance to calcium dichloride: decreased resistance to caffeine: decreased resistance to hygromycin B: decreased resistance to sorbate: decreased respiratory growth: decreased rate sporulation: decreased toxin resistance: decreased vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	513 total interaction(s) for 294 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 2 Affinity Capture-Western: 6 Co-crystal Structure: 1 PCA: 21 Protein-peptide: 10 Reconstituted Complex: 8 Two-hybrid: 25
Genetic Interactions	Dosage Growth Defect: 3 Dosage Lethality: 5 Dosage Rescue: 4 Negative Genetic: 273 Positive Genetic: 25 Synthetic Growth Defect: 69 Synthetic Lethality: 47 Synthetic Rescue: 6
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:781100 to 780390 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..711 781100..780390 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002569