YCF1/YDR135C Summary

YCF1/YDR135C Summary

Standard Name	YCF1 1
Systematic Name	YDR135C
Feature Type	ORF, Verified
Description	Vacuolar glutathione S-conjugate transporter; member of ATP-binding cassette family; ATPase activity required to support vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; has role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR (1, 2, 3, 4, 5, 6, 7)
Name Description	Yeast Cadmium Factor 1

Chromosomal Location	ChrIV:727551 to 723004 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All YCF1 GO evidence and references

	View Computational GO annotations for YCF1
Molecular Function
Manually curated	bilirubin transmembrane transporter activity (IGI, IMP) glutathione S-conjugate-exporting ATPase activity (IDA)
Biological Process
Manually curated	bilirubin transport (IGI, IMP) response to metal ion (IDA) transmembrane transport (IMP) vacuole fusion, non-autophagic (IMP)
Cellular Component
Manually curated	fungal-type vacuole membrane (IDA)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All YCF1 Phenotype evidence and references

Classical genetics
activation	metal resistance: increased
null	cell size: increased metal resistance: decreased metal resistance: increased oxidative stress resistance: decreased resistance to antimonite: decreased resistance to arsenite(3-): decreased
overexpression	resistance to diazaborine: increased
reduction of function	metal resistance: decreased
Large-scale survey
null	budding index: increased competitive fitness: decreased haploinsufficient resistance to cadmium dichloride: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to sodium arsenite: decreased resistance to glycolaldehyde: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All YCF1 Interaction evidence and references

	45 total interaction(s) for 28 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 FRET: 1 PCA: 9 Reconstituted Complex: 1 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 5 Phenotypic Enhancement: 5 Synthetic Growth Defect: 6 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All YCF1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:727551 to 723004 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4548	727551..723004	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| TCDB \| UniProtKB

Primary SGDID	S000002542

References cited on this page View Complete Literature Guide for YCF1

1)	Szczypka MS, et al. (1994) A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein. J Biol Chem 269(36):22853-7
2)	Petrovic S, et al. (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71
3)	Li ZS, et al. (1996) The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump. J Biol Chem 271(11):6509-17
4)	Li ZS, et al. (1997) A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium. Proc Natl Acad Sci U S A 94(1):42-7
5)	Gueldry O, et al. (2003) Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem 270(11):2486-96
6)	Lazard M, et al. (2011) Selenodiglutathione uptake by the Saccharomyces cerevisiae vacuolar ATP-binding cassette transporter Ycf1p. FEBS J 278(21):4112-21
7)	Sasser TL, et al. (2013) The Yeast ABC Transporter Ycf1p Enhances the Recruitment of the Soluble SNARE Vam7p to Vacuoles for Efficient Membrane Fusion. J Biol Chem ()