YCF1/YDR135C Summary Help

Standard Name YCF1 1
Systematic Name YDR135C
Feature Type ORF, Verified
Description Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR (1, 2, 3, 4, 5, 6, 7, 8)
Name Description Yeast Cadmium Factor 1
Chromosomal Location
ChrIV:727551 to 723004 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All YCF1 GO evidence and references
  View Computational GO annotations for YCF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
94 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • FRET: 1
  • PCA: 38
  • Reconstituted Complex: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 13
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 6
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 1,515
Molecular Weight (Da) 171,120
Isoelectric Point (pI) 8.49
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:727551 to 723004 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..4548 727551..723004 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002542
References cited on this page View Complete Literature Guide for YCF1
1) Szczypka MS, et al.  (1994) A yeast metal resistance protein similar to human cystic fibrosis transmembrane conductance regulator (CFTR) and multidrug resistance-associated protein. J Biol Chem 269(36):22853-7
2) Petrovic S, et al.  (2000) The products of YCF1 and YLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin in Saccharomyces cerevisiae. Yeast 16(6):561-71
3) Li ZS, et al.  (1996) The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump. J Biol Chem 271(11):6509-17
4) Li ZS, et al.  (1997) A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium. Proc Natl Acad Sci U S A 94(1):42-7
5) Gueldry O, et al.  (2003) Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur J Biochem 270(11):2486-96
6) Lazard M, et al.  (2011) Selenodiglutathione uptake by the Saccharomyces cerevisiae vacuolar ATP-binding cassette transporter Ycf1p. FEBS J 278(21):4112-21
7) Morgan B, et al.  (2013) Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis. Nat Chem Biol 9(2):119-25
8) Sasser TL, et al.  (2013) The Yeast ATP-binding Cassette (ABC) Transporter Ycf1p Enhances the Recruitment of the Soluble SNARE Vam7p to Vacuoles for Efficient Membrane Fusion. J Biol Chem 288(25):18300-10