FIN1/YDR130C Summary Help

Standard Name FIN1 1
Systematic Name YDR130C
Feature Type ORF, Verified
Description Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description Filaments In between Nuclei 1
Chromosomal Location
ChrIV:716622 to 715747 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All FIN1 GO evidence and references
  View Computational GO annotations for FIN1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
overexpression
Resources
166 total interaction(s) for 123 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 91
  • Biochemical Activity: 7
  • Co-crystal Structure: 1
  • Protein-peptide: 1
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 27
  • Phenotypic Enhancement: 1
  • Positive Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 291
Molecular Weight (Da) 33,186
Isoelectric Point (pI) 10.6
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:716622 to 715747 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..876 716622..715747 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002537
SUMMARY PARAGRAPH for FIN1

Fin1p is a spindle pole body-related intermediate filament protein that forms cell cycle-specific filaments between spindle pole bodies in mother and daughter cells, and is able to self-assemble in vitro (1, 3).

FIN1 expression is induced during the S/G2 phase of the cell cycle (2), and Fin1p is a Clb5p-Cdc28p specific substrate that is fully phosphorylated immediately after its synthesis in early S phase. The protein is then dephosphorylated after degradation of Clb5p during mitosis (7). This Fin1p dephosphorylation is dependent on Bmh1p and Bmh2p, as the protein cannot be dephosphorylated in a bmh1 bmh2 double null mutant (2). Fin1p has higher affinity for Bmh2p than for Bmh1p (3), and Bmh2p only interacts with phosphorylated forms of Fin1p (2). Phosphorylated Fin1p also interacts with the Glc7p catalytic subunit of the protein phosphatase type 1 complex, suggesting that Glc7p and Bmh1p/Bmh2p are involved in regulating the phosphorylation state of Fin1p (2).

In resting cells Fin1p is undetectable, in small-budded cells Fin1p is localized in the nucleus, and during late mitosis Fin1p localizes to the spindle pole bodies (1). fin1 null mutants are viable on both minimal and rich growth media. Overexpression of FIN1 in haploid cells is lethal, and in diploid cells it results in very poor growth, altered cell morphology, and an accumulation of filamentous structures that resemble the neurofibrillary tangles found in cells of patients with Alzheimer's disease (1).

Last updated: 2005-10-07 Contact SGD

References cited on this page View Complete Literature Guide for FIN1
1) van Hemert MJ, et al.  (2002) The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies. Proc Natl Acad Sci U S A 99(8):5390-3
2) Mayordomo I and Sanz P  (2002) The Saccharomyces cerevisiae 14-3-3 protein Bmh2 is required for regulation of the phosphorylation status of Fin1, a novel intermediate filament protein. Biochem J 365(Pt 1):51-6
3) van Hemert MJ, et al.  (2003) Self-association of the spindle pole body-related intermediate filament protein Fin1p and its phosphorylation-dependent interaction with 14-3-3 proteins in yeast. J Biol Chem 278(17):15049-55
4) Woodbury EL and Morgan DO  (2007) The Role of Self-association in Fin1 Function on the Mitotic Spindle. J Biol Chem 282(44):32138-43
5) Akiyoshi B, et al.  (2009) Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit. Genes Dev 23(24):2887-99
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Loog M and Morgan DO  (2005) Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature 434(7029):104-8