SAC6/YDR129C Summary Help

Standard Name SAC6 1, 2
Systematic Name YDR129C
Alias ABP67 3
Feature Type ORF, Verified
Description Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress (4, 5, 6, 7, 8)
Name Description Suppressor of ACtin 9
Chromosomal Location
ChrIV:715379 to 713340 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 84 cM
Gene Ontology Annotations All SAC6 GO evidence and references
  View Computational GO annotations for SAC6
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
overexpression
Large-scale survey
null
Resources
162 total interaction(s) for 101 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 36
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 2
  • Co-fractionation: 2
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 5
  • Reconstituted Complex: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 13
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 23
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 43
  • Synthetic Rescue: 11

Resources
Expression Summary
histogram
Resources
Length (a.a.) 642
Molecular Weight (Da) 71,772
Isoelectric Point (pI) 5.22
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:715379 to 713340 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 84 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..21 715379..715359 2011-02-03 1996-07-31
Intron 22..132 715358..715248 2011-02-03 1996-07-31
CDS 133..2040 715247..713340 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002536
References cited on this page View Complete Literature Guide for SAC6
1) Adams AE and Botstein D  (1989) Dominant suppressors of yeast actin mutations that are reciprocally suppressed. Genetics 121(4):675-83
2) Adams, A., et al.  (1989) Personal Communication, Mortimer Map Edition 10
3) Adams AE, et al.  (1989) A yeast actin-binding protein is encoded by SAC6, a gene found by suppression of an actin mutation. Science 243(4888):231-3
4) Karpova TS, et al.  (1995) Actin filaments in yeast are unstable in the absence of capping protein or fimbrin. J Cell Biol 131(6 Pt 1):1483-93
5) Adams AE, et al.  (1995) Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin. Mol Cell Biol 15(1):69-75
6) Goodman A, et al.  (2003) The Saccharomyces cerevisiae calponin/transgelin homolog Scp1 functions with fimbrin to regulate stability and organization of the actin cytoskeleton. Mol Biol Cell 14(7):2617-29
7) Winder SJ, et al.  (2003) SCP1 encodes an actin-bundling protein in yeast. Biochem J 375(Pt 2):287-95
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Novick P, et al.  (1989) Suppressors of yeast actin mutations. Genetics 121(4):659-74