INO2/YDR123C Summary Help

Standard Name INO2 1
Systematic Name YDR123C
Alias DIE1 , SCS1 2
Feature Type ORF, Verified
Description Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift (3, 4, 5, 6, 7)
Name Description INOsitol requiring 1
Chromosomal Location
ChrIV:699468 to 698554 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All INO2 GO evidence and references
  View Computational GO annotations for INO2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 77 genes
Regulators 9 genes
Classical genetics
Large-scale survey
265 total interaction(s) for 162 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 6
  • Biochemical Activity: 1
  • Co-localization: 1
  • Far Western: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 186
  • Phenotypic Enhancement: 2
  • Positive Genetic: 33
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 2
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 304
Molecular Weight (Da) 34,234
Isoelectric Point (pI) 6.23
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:699468 to 698554 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..915 699468..698554 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002530
References cited on this page View Complete Literature Guide for INO2
1) Loewy BS and Henry SA  (1984) The INO2 and INO4 loci of Saccharomyces cerevisiae are pleiotropic regulatory genes. Mol Cell Biol 4(11):2479-85
2) Hosaka K, et al.  (1994) Cloning and characterization of the SCS1 gene required for the expression of genes in yeast phospholipid synthesis. J Biochem (Tokyo) 115(1):131-6
3) Schuller HJ, et al.  (1995) DNA binding site of the yeast heteromeric Ino2p/Ino4p basic helix-loop-helix transcription factor: structural requirements as defined by saturation mutagenesis. FEBS Lett 370(1-2):149-52
4) Hammond CL, et al.  (1993) INO2, a regulatory gene in yeast phospholipid biosynthesis, affects nuclear segregation and bud pattern formation. Cell Mol Biol Res 39(6):561-77
5) Ambroziak J and Henry SA  (1994) INO2 and INO4 gene products, positive regulators of phospholipid biosynthesis in Saccharomyces cerevisiae, form a complex that binds to the INO1 promoter. J Biol Chem 269(21):15344-9
6) Gardenour KR, et al.  (2004) Identification of novel dominant INO2c mutants with an Opi- phenotype. Mol Microbiol 52(5):1271-80
7) Zampar GG, et al.  (2013) Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast. Mol Syst Biol 9():651
8) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104