INO2/YDR123C Summary Help

INO2 BASIC INFORMATION

Standard Name INO2
Systematic Name YDR123C
Alias DIE1 , SCS1 1
Feature Type ORF, Verified
Description Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion (2, 3, 4, 5)
Name Description INOsitol requiring 6
GO Annotations All INO2 GO evidence and references
    View Computational GO annotations for INO2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs INO2 Transcription Factor Binding Sites and References
  • CACATGC
  • CATGTGAAAT
Regulatory modules predicted: stressResponse ( 391 )
Mutant Phenotype All INO2 Phenotype details and references
Classical genetics
overexpression
Large-scale survey
null
overexpression
Interactions INO2 All interactions details and references
32 total interaction(s) for 14 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • Far Western: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 7
Genetic Interactions
  • Dosage Rescue: 4
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2
Sequence Information
ChrIV:699466 to 698552 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2008-06-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..915 699466..698552 2008-06-05 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002530

INO2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for INO2

NOMENCLATURE CONFLICT NOTE

NameRelevanceDescription
LCB2Nomenclature conflictSCS1 has been used to describe both INO2/YDR123C, a transcription factor involved in phospholipid synthesis, and LCB2/YDR062W, a serine C-palmitoyltransferase involved in sphingolipid biosynthesis.
RTS1Nomenclature conflictSCS1 has been used in the literature to refer to both RTS1/YOR014W, which encodes a protein phosphatase 2A and INO2/YDR123C, which encodes a RNA Pol II transcription factor.

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for INO2]

1) Hosaka K, et al.  (1994) Cloning and characterization of the SCS1 gene required for the expression of genes in yeast phospholipid synthesis. J Biochem 115(1):131-6
2) Schuller HJ, et al.  (1995) DNA binding site of the yeast heteromeric Ino2p/Ino4p basic helix-loop-helix transcription factor: structural requirements as defined by saturation mutagenesis. FEBS Lett 370(1-2):149-52
3) Hammond CL, et al.  (1993) INO2, a regulatory gene in yeast phospholipid biosynthesis, affects nuclear segregation and bud pattern formation. Cell Mol Biol Res 39(6):561-77
4) Ambroziak J and Henry SA  (1994) INO2 and INO4 gene products, positive regulators of phospholipid biosynthesis in Saccharomyces cerevisiae, form a complex that binds to the INO1 promoter. J Biol Chem 269(21):15344-9
5) Gardenour KR, et al.  (2004) Identification of novel dominant INO2c mutants with an Opi- phenotype. Mol Microbiol 52(5):1271-80
6) Loewy BS and Henry SA  (1984) The INO2 and INO4 loci of Saccharomyces cerevisiae are pleiotropic regulatory genes. Mol Cell Biol 4(11):2479-85