TRM1/YDR120C Summary Help

Standard Name TRM1
Systematic Name YDR120C
Feature Type ORF, Verified
Description tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments (1, 2, 3)
Name Description Transfer RNA Methyltransferase
Chromosomal Location
ChrIV:693261 to 691549 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All TRM1 GO evidence and references
  View Computational GO annotations for TRM1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
124 total interaction(s) for 95 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 3
  • Biochemical Activity: 7
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 75
  • Positive Genetic: 7
  • Synthetic Growth Defect: 15
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 570
Molecular Weight (Da) 64,052
Isoelectric Point (pI) 9.79
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:693261 to 691549 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1713 693261..691549 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002527
References cited on this page View Complete Literature Guide for TRM1
1) Ellis SR, et al.  (1989) Amino-terminal extension generated from an upstream AUG codon increases the efficiency of mitochondrial import of yeast N2,N2-dimethylguanosine-specific tRNA methyltransferases. Mol Cell Biol 9(4):1611-20
2) Ellis SR, et al.  (1986) Isolation and characterization of the TRM1 locus, a gene essential for the N2,N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNA in Saccharomyces cerevisiae. J Biol Chem 261(21):9703-9
3) Rose AM, et al.  (1992) Separate information required for nuclear and subnuclear localization: additional complexity in localizing an enzyme shared by mitochondria and nuclei. Mol Cell Biol 12(12):5652-8