Standard Name	TRM1
Systematic Name	YDR120C
Feature Type	ORF, Verified
Description	tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments (1, 2, 3)
Name Description	Transfer RNA Methyltransferase

Chromosomal Location	ChrIV:693261 to 691549 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for TRM1
Molecular Function
Manually curated	tRNA (guanine-N2-)-methyltransferase activity (IDA, IMP)
Biological Process
Manually curated	tRNA methylation (IMP)
Cellular Component
Manually curated	mitochondrion (IDA) nuclear envelope (IDA) nuclear inner membrane (IDA, IMP)

Classical genetics
null	heat sensitivity: increased
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased Msb2p-HA secretion: decreased resistance to benomyl: decreased resistance to fenpropimorph: increased resistance to 5-fluorouracil: decreased resistance to cycloheximide: decreased resistance to tunicamycin: decreased resistance to bleomycin: increased respiratory growth: decreased rate utilization of nitrogen source: absent viable
overexpression	vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	123 total interaction(s) for 95 unique genes/features.
Physical Interactions	Affinity Capture-MS: 9 Affinity Capture-RNA: 3 Biochemical Activity: 7 PCA: 1 Reconstituted Complex: 1 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 75 Positive Genetic: 7 Synthetic Growth Defect: 15 Synthetic Lethality: 3 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:693261 to 691549 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1713 693261..691549 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002527