TRS85/YDR108W Summary Help

Standard Name TRS85 1
Systematic Name YDR108W
Alias MUM1 2 , GSG1 3
Feature Type ORF, Verified
Description Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role (2, 3, 4, 5, 6)
Name Description TRapp Subunit 1
Chromosomal Location
ChrIV:671269 to 673365 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All TRS85 GO evidence and references
  View Computational GO annotations for TRS85
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 42 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
Resources
557 total interaction(s) for 272 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 34
  • Affinity Capture-Western: 16
  • Co-fractionation: 1
  • Co-localization: 1
  • PCA: 3
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 342
  • Phenotypic Enhancement: 68
  • Phenotypic Suppression: 14
  • Positive Genetic: 46
  • Synthetic Growth Defect: 18
  • Synthetic Lethality: 8

Resources
Expression Summary
histogram
Resources
Length (a.a.) 698
Molecular Weight (Da) 80,494
Isoelectric Point (pI) 6.38
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:671269 to 673365 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2097 671269..673365 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002515
References cited on this page View Complete Literature Guide for TRS85
1) Sacher M, et al.  (2000) Identification and characterization of five new subunits of TRAPP. Eur J Cell Biol 79(2):71-80
2) Engebrecht J, et al.  (1998) Yeast meiotic mutants proficient for the induction of ectopic recombination. Genetics 148(2):581-98
3) Kaytor MD and Livingston DM  (1995) GSG1, a yeast gene required for sporulation. Yeast 11(12):1147-55
4) Meiling-Wesse K, et al.  (2005) Trs85 (Gsg1), a component of the TRAPP complexes, is required for the organization of the preautophagosomal structure during selective autophagy via the Cvt pathway. J Biol Chem 280(39):33669-78
5) Nazarko TY, et al.  (2005) Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae. Autophagy 1(1):37-45
6) Lynch-Day MA, et al.  (2010) Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy. Proc Natl Acad Sci U S A 107(17):7811-6