GIS1/YDR096W Summary

GIS1 BASIC INFORMATION

Standard Name	GIS1
Systematic Name	YDR096W
Feature Type	ORF, Verified
Description	JmjC domain-containing histone demethylase; transcription factor involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1 (1, 2, 3, 4, 5)
Name Description	GIg1-2 Suppressor 3

GO Annotations	All GIS1 GO evidence and references
	View Computational GO annotations for GIS1
Molecular Function
Manually curated	DNA binding (IDA) histone demethylase activity (H3-K36 specific) (IMP) specific transcriptional repressor activity (IDA, IGI, IMP) transcription activator activity (IDA, IGI, IMP)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	ascospore wall assembly (IMP) histone demethylation (IMP) phospholipid metabolic process (IMP)
Cellular Component
High-throughput	mitochondrion (IDA) nucleus (IDA)

Mutant Phenotype	All GIS1 Phenotype details and references
Classical genetics
null	Spr1-GFP distribution: abnormal spore wall formation: abnormal utilization of carbon source: decreased
overexpression	cell size: abnormal cellular morphology: abnormal
Large-scale survey
null	sporulation: normal viable
overexpression	vegetative growth: decreased

Interactions	GIS1 All interactions details and references
	50 total interaction(s) for 40 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Affinity Capture-Western: 3 Two-hybrid: 19
Genetic Interactions	Dosage Lethality: 3 Dosage Rescue: 4 Phenotypic Enhancement: 9 Phenotypic Suppression: 4 Synthetic Growth Defect: 5

Sequence Information

ChrIV:637137 to 639821 | |

Last Update

Coordinates: 2008-06-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2685	637137..639821	2008-06-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000002503

GIS1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for GIS1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GIS1]

1)	Jang YK, et al. (1999) RPH1 and GIS1 are damage-responsive repressors of PHR1. Mol Cell Biol 19(11):7630-8
2)	Pedruzzi I, et al. (2000) Saccharomyces cerevisiae Ras/cAMP pathway controls post-diauxic shift element-dependent transcription through the zinc finger protein Gis1. EMBO J 19(11):2569-79
3)	Balciunas D and Ronne H (1999) Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells. Mol Gen Genet 262(4-5):589-99
4)	Oshiro J, et al. (2003) Regulation of the yeast DPP1-encoded diacylglycerol pyrophosphate phosphatase by transcription factor Gis1p. J Biol Chem 278(34):31495-503
5)	Tu S, et al. (2007) Identification of histone demethylases in Saccharomyces cerevisiae. J Biol Chem 282(19):14262-71

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