UBC13/YDR092W Summary Help

Standard Name UBC13 1
Systematic Name YDR092W
Feature Type ORF, Verified
Description E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus (2, 3, 4)
Name Description UBiquitin-Conjugating 5
Chromosomal Location
ChrIV:629876 to 630605 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All UBC13 GO evidence and references
  View Computational GO annotations for UBC13
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
194 total interaction(s) for 131 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 13
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 3
  • PCA: 7
  • Reconstituted Complex: 8
  • Two-hybrid: 11

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 96
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 3
  • Positive Genetic: 11
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 3
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 153
Molecular Weight (Da) 17,468
Isoelectric Point (pI) 5.15
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:629876 to 630605 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..30 629876..629905 2011-02-03 1996-07-31
Intron 31..298 629906..630173 2011-02-03 1996-07-31
CDS 299..730 630174..630605 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002499
References cited on this page View Complete Literature Guide for UBC13
1) Jelinsky SA and Samson LD  (1999) Global response of Saccharomyces cerevisiae to an alkylating agent. Proc Natl Acad Sci U S A 96(4):1486-91
2) Ulrich HD and Jentsch S  (2000) Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair. EMBO J 19(13):3388-97
3) Brusky J, et al.  (2000) UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae. Curr Genet 37(3):168-74
4) Hofmann RM and Pickart CM  (1999) Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Cell 96(5):645-53
5) Seufert W, et al.  (1990) UBC1 encodes a novel member of an essential subfamily of yeast ubiquitin-conjugating enzymes involved in protein degradation. EMBO J 9(13):4535-41