AFR1/YDR085C Summary Help

AFR1 BASIC INFORMATION

Standard Name AFR1
Systematic Name YDR085C
Feature Type ORF, Verified
Description Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p (1, 2, 3, 4, 5)
Name Description Alpha-Factor Receptor regulator 6
GO Annotations All AFR1 GO evidence and references
    View Computational GO annotations for AFR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Regulatory modules predicted: stressResponse (607, 481, 457, 451)
Mutant Phenotype All AFR1 Phenotype details and references
Classical genetics
null
reduction of function
Large-scale survey
null
Interactions AFR1 All interactions details and references
  View additional details at BioGRID
23 total interaction(s) for 17 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • FRET: 2
  • Protein-RNA: 1
  • Two-hybrid: 9
Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 2
  • Positive Genetic: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1
Sequence Information
ChrIV:616148 to 614286 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 47.97 cM
Last Update Coordinates: 2008-06-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1863 616148..614286 2008-06-05 1996-07-31
Post-translational Modifications PhosphoGRID | PhosphoPep Database
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000002492

AFR1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for AFR1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for AFR1]

1) DeMattei CR, et al.  (2000) Point mutations identify a conserved region of the saccharomyces cerevisiae AFR1 gene that is essential for both the pheromone signaling and morphogenesis functions. Genetics 155(1):43-55
2) Konopka JB  (1993) AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation. Mol Cell Biol 13(11):6876-88
3) Giot L and Konopka JB  (1997) Functional analysis of the interaction between Afr1p and the Cdc12p septin, two proteins involved in pheromone-induced morphogenesis. Mol Biol Cell 8(6):987-98
4) Changwei Z, et al.  (2007) Afr1p has a role in regulating the localization of Mpk1p at the shmoo tip in Saccharomyces cerevisiae. FEBS Lett 581(14):2670-4
5) Bharucha JP, et al.  (2008) Saccharomyces cerevisiae Afr1 protein is a protein phosphatase 1/Glc7-targeting subunit that regulates the septin cytoskeleton during mating. Eukaryot Cell 7(8):1246-55
6) Davis C, et al.  (1998) Afr1p regulates the Saccharomyces cerevisiae alpha-factor receptor by a mechanism that is distinct from receptor phosphorylation and endocytosis. Genetics 148(2):625-35