AFR1/YDR085C Summary Help

Standard Name AFR1 1
Systematic Name YDR085C
Feature Type ORF, Verified
Description Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Alpha-Factor Receptor regulator 7
Chromosomal Location
ChrIV:616150 to 614288 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 47.97 cM
Gene Ontology Annotations All AFR1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Classical genetics
reduction of function
Large-scale survey
25 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • FRET: 2
  • Protein-RNA: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 2
  • Positive Genetic: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 620
Molecular Weight (Da) 71,363
Isoelectric Point (pI) 9.23
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:616150 to 614288 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 47.97 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1863 616150..614288 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002492
References cited on this page View Complete Literature Guide for AFR1
1) Konopka JB  (1993) AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation. Mol Cell Biol 13(11):6876-88
2) DeMattei CR, et al.  (2000) Point mutations identify a conserved region of the saccharomyces cerevisiae AFR1 gene that is essential for both the pheromone signaling and morphogenesis functions. Genetics 155(1):43-55
3) Giot L and Konopka JB  (1997) Functional analysis of the interaction between Afr1p and the Cdc12p septin, two proteins involved in pheromone-induced morphogenesis. Mol Biol Cell 8(6):987-98
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Changwei Z, et al.  (2007) Afr1p has a role in regulating the localization of Mpk1p at the shmoo tip in Saccharomyces cerevisiae. FEBS Lett 581(14):2670-4
6) Bharucha JP, et al.  (2008) Saccharomyces cerevisiae Afr1 protein is a protein phosphatase 1/Glc7-targeting subunit that regulates the septin cytoskeleton during mating. Eukaryot Cell 7(8):1246-55
7) Davis C, et al.  (1998) Afr1p regulates the Saccharomyces cerevisiae alpha-factor receptor by a mechanism that is distinct from receptor phosphorylation and endocytosis. Genetics 148(2):625-35