RRP8/YDR083W Summary Help

Standard Name RRP8
Systematic Name YDR083W
Feature Type ORF, Verified
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 (1, 2, 3, 4)
Name Description Ribosomal RNA Processing
Chromosomal Location
ChrIV:612073 to 613251 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All RRP8 GO evidence and references
  View Computational GO annotations for RRP8
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All RRP8 Phenotype evidence and references
Classical genetics
null
reduction of function
Large-scale survey
null
Resources
interactions All RRP8 Interaction evidence and references
139 total interaction(s) for 110 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 2
Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 70
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 46
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All RRP8 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:612073 to 613251 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2006-01-19
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1179 612073..613251 2011-02-03 2006-01-19
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002490
References cited on this page View Complete Literature Guide for RRP8
1) Bousquet-Antonelli C, et al.  (2000) Rrp8p is a yeast nucleolar protein functionally linked to Gar1p and involved in pre-rRNA cleavage at site A2. RNA 6(6):826-43
2) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
3) Ben-Shitrit T, et al.  (2012) Systematic identification of gene annotation errors in the widely used yeast mutation collections.LID - 10.1038/nmeth.1890 [doi] Nat Methods ()
4) Peifer C, et al.  (2013) Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA. Nucleic Acids Res 41(2):1151-63