PET100/YDR079W Summary Help

Standard Name PET100
Systematic Name YDR079W
Feature Type ORF, Verified
Description Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme (1, 2 and see Summary Paragraph)
Name Description PETite colonies
Chromosomal Location
ChrIV:603064 to 603399 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All PET100 GO evidence and references
  View Computational GO annotations for PET100
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
9 total interaction(s) for 9 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3

Genetic Interactions
  • Synthetic Growth Defect: 2
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 111
Molecular Weight (Da) 13,187
Isoelectric Point (pI) 10.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:603064 to 603399 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..336 603064..603399 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002486
SUMMARY PARAGRAPH for PET100

PET100 encodes an integral protein of the mitochondrial inner membrane protein that is required for assembly of cytochrome c oxidase (1, 2). Pet100p acts as a chaperone to specifically mediate the assembly of a Cox7p-Cox8p-Cox9p subcomplex (subunits VII, VIII, and VIIa respectively) into the cytochrome c oxidase holoenzyme (1). Protein-protein interactions are detected between Pet100p and this subcomplex, but not between Pet100p and the holoenzyme (2).

The pet100 null mutant displays a respiratory growth defect and lacks cytochrome c oxidase activity although all of the subunits are synthesized (3). Two cytochrome c oxidase subcomplexes accumulate in a pet100 null mutant: one containing Cox7p, Cox8p, and Cox9p (subunits VII, VIII, and VIIa) and another containing Cox5Ap and Cox6p (subunits Va and VI) (2).

Last updated: 2007-01-08 Contact SGD

References cited on this page View Complete Literature Guide for PET100
1) Forsha D, et al.  (2001) Structure and function of Pet100p, a molecular chaperone required for the assembly of cytochrome c oxidase in Saccharomyces cerevisiae. Biochem Soc Trans 29(Pt 4):436-41
2) Church C, et al.  (2005) A role for Pet100p in the assembly of yeast cytochrome c oxidase: interaction with a subassembly that accumulates in a pet100 mutant. J Biol Chem 280(3):1854-63
3) Church C, et al.  (1996) Cloning and characterization of PET100, a gene required for the assembly of yeast cytochrome c oxidase. J Biol Chem 271(31):18499-507