MAK21/YDR060W Summary Help

Standard Name MAK21 1
Systematic Name YDR060W
Alias NOC1 2
Feature Type ORF, Verified
Description Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein (1, 3, 4)
Name Description MAintenance of Killer 5
Chromosomal Location
ChrIV:570649 to 573726 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All MAK21 GO evidence and references
  View Computational GO annotations for MAK21
Molecular Function
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
repressible
Large-scale survey
null
overexpression
reduction of function
repressible
Resources
304 total interaction(s) for 173 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 239
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 11
  • Co-fractionation: 2
  • PCA: 3
  • Reconstituted Complex: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 30
  • Phenotypic Suppression: 6
  • Positive Genetic: 3
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,025
Molecular Weight (Da) 116,675
Isoelectric Point (pI) 4.72
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:570649 to 573726 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3078 570649..573726 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002467
References cited on this page View Complete Literature Guide for MAK21
1) Edskes HK, et al.  (1998) Mak21p of Saccharomyces cerevisiae, a homolog of human CAATT-binding protein, is essential for 60 S ribosomal subunit biogenesis. J Biol Chem 273(44):28912-20
2) Milkereit P, et al.  (2001) Maturation and intranuclear transport of pre-ribosomes requires Noc proteins. Cell 105(4):499-509
3) Horsey EW, et al.  (2004) Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10(5):813-27
4) Hierlmeier T, et al.  (2013) Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae. Nucleic Acids Res 41(2):1191-210
5) Wickner RB  (1974) Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics 76(3):423-32