Standard Name	NRG1 1
Systematic Name	YDR043C
Feature Type	ORF, Verified
Description	Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Negative Regulator of Glucose-repressed genes 1, 2

Chromosomal Location	ChrIV:543369 to 542674 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for NRG1
Molecular Function
Manually curated	RNA polymerase II core promoter proximal region sequence-specific DNA binding (IDA, ISM) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription (IDA, IMP) RNA polymerase II repressing transcription factor binding (IPI) RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription (IGI)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	biofilm formation (IGI, IMP) cellular hyperosmotic salinity response (IMP, IPI) negative regulation of cellular hyperosmotic salinity response by negative regulation of transcription from RNA polymerase II promoter (IGI) negative regulation of cellular response to alkalinity by negative regulation of transcription from RNA polymerase II promoter (IMP, IPI) negative regulation of invasive growth in response to glucose limitation by negative regulation of transcription from RNA polymerase II promoter (IGI) negative regulation of transcription from RNA polymerase II promoter (IMP) negative regulation of transcription from RNA polymerase II promoter by glucose (IGI, IMP) pseudohyphal growth (IGI)
Cellular Component
Manually curated	nucleus (IDA)

Binding motifs	Predicted NRG1 Binding Site Locations
	CCCT 2 [Search intergenic regions for CCCT] GGACCCT 6 [Search intergenic regions for GGACCCT] MAGGGTCC 7 [Search intergenic regions for MAGGGTCC]
Regulatory modules	predicted: cellcycle (389)
Resources	ScerTF | UniProbe | YEASTRACT | YeTFaSCo

Classical genetics
null	lithium(1+) accumulation: decreased ionic stress resistance: increased Suc2p activity: increased resistance to capric acid: decreased resistance to acetic acid: decreased
overexpression	Suc2p activity: decreased utilization of carbon source: decreased
Large-scale survey
null	auxotrophy polyphosphate accumulation: abnormal competitive fitness: increased resistance to benzo[a]pyrene: decreased resistance to nickel sulfate: decreased stress resistance: decreased toxin resistance: increased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: decreased vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	176 total interaction(s) for 143 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Biochemical Activity: 2 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 8 Dosage Rescue: 1 Negative Genetic: 103 Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Positive Genetic: 35 Synthetic Growth Defect: 2 Synthetic Rescue: 11
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:543369 to 542674 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..696 543369..542674 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002450