ENA1/YDR040C Summary Help

Standard Name ENA1 1
Systematic Name YDR040C
Alias HOR6 2 , PMR2 3
Feature Type ORF, Verified
Description P-type ATPase sodium pump; involved in Na+ and Li+ efflux to allow salt tolerance (1, 4 and see Summary Paragraph)
Name Description Exitus NAtru (Latin, "exit sodium") 1
Chromosomal Location
ChrIV:538467 to 535192 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 15 cM
Gene Ontology Annotations All ENA1 GO evidence and references
  View Computational GO annotations for ENA1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Classical genetics
Large-scale survey
53 total interaction(s) for 35 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Co-fractionation: 2
  • Protein-RNA: 1

Genetic Interactions
  • Dosage Rescue: 16
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 13
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 1,091
Molecular Weight (Da) 120,356
Isoelectric Point (pI) 5.32
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:538467 to 535192 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 15 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3276 538467..535192 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002447

ENA1 is the first member of a tandem array of genes encoding nearly, but not perfectly, identical P-Type ATPases. The reference strain, S288c, contains three genes in this cluster: ENA1, ENA2, and ENA5. Other Saccharomyces strains typically contain 4 or 5 genes (ENA1-ENA5) (3, 5).

ENA1 is the most well characterized member of this cluster and is thought to encode the primary plasma membrane Na+-ATPase exporter in S. cerevisiae. Ena1p plays a critical role in the detoxification of Na+ ions and in maintaining ion homeostasis, making Ena1p a principal component of the cell's ability to survive high salt or alkaline conditions (6). Ena1p and Ena2p both transport Li+ ions, but with differing efficiencies (3), and have been shown to export K+ ions concomitantly with Na+, at some salt concentrations (7).

Although basal levels of ENA1 expression are very low, it is rapidly induced in response to osmotic, saline, and alkaline stresses. Transcriptional regulation of ENA1 is complex and is independently mediated by multiple signaling pathways (6) including the HOG1 pathway (8), the Calcineurin pathway (8), the TOR pathway (9), the RIM101 pathway (10), and the glucose repression pathway (11).

Ena1p-Ena5p are closely related to Pmr1p (3), the Golgi membrane Ca2+-ATPase. Mutations in the human homolog of Pmr1p, ATP2C1, cause the acantholytic skin condition Hailey-Hailey disease (12).

Last updated: 2006-10-31 Contact SGD

References cited on this page View Complete Literature Guide for ENA1
1) Haro R, et al.  (1991) A novel P-type ATPase from yeast involved in sodium transport. FEBS Lett 291(2):189-91
2) Hirayama T, et al.  (1995) Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae. Mol Gen Genet 249(2):127-38
3) Wieland J, et al.  (1995) The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na+ pump in the yeast plasma membrane. EMBO J 14(16):3870-82
4) Garciadeblas B, et al.  (1993) Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in Saccharomyces cerevisiae. Mol Gen Genet 236(2-3):363-8
5) Martinez R, et al.  (1991) A PMR2 tandem repeat with a modified C-terminus is located downstream from the KRS1 gene encoding lysyl-tRNA synthetase in Saccharomyces cerevisiae. Mol Gen Genet 227(1):149-54
6) Platara M, et al.  (2006) The Transcriptional Response of the Yeast Na+-ATPase ENA1 Gene to Alkaline Stress Involves Three Main Signaling Pathways. J Biol Chem 281(48):36632-42
7) Benito B, et al.  (2002) Potassium- or sodium-efflux ATPase, a key enzyme in the evolution of fungi. Microbiology 148(Pt 4):933-41
8) Marquez JA and Serrano R  (1996) Multiple transduction pathways regulate the sodium-extrusion gene PMR2/ENA1 during salt stress in yeast. FEBS Lett 382(1-2):89-92
9) Crespo JL, et al.  (2001) The GATA transcription factors GLN3 and GAT1 link TOR to salt stress in Saccharomyces cerevisiae. J Biol Chem 276(37):34441-4
10) Serrano R, et al.  (2002) The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling. Mol Microbiol 46(5):1319-33
11) Alepuz PM, et al.  (1997) Glucose repression affects ion homeostasis in yeast through the regulation of the stress-activated ENA1 gene. Mol Microbiol 26(1):91-8
12) Marie Mauro T  (2004) Yeast researchers consider Hailey-Hailey disease. J Invest Dermatol 123(6):xxii-xxiii