ENA2/YDR039C Summary Help

Standard Name ENA2 1
Systematic Name YDR039C
Feature Type ORF, Verified
Description P-type ATPase sodium pump; involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux (1, 2 and see Summary Paragraph)
Name Description Exitus NAtru (Latin, "exit sodium") 1
Chromosomal Location
ChrIV:534582 to 531307 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ENA2 GO evidence and references
  View Computational GO annotations for ENA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Classical genetics
Large-scale survey
23 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Co-fractionation: 2
  • PCA: 5
  • Protein-RNA: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 1

Expression Summary
Length (a.a.) 1,091
Molecular Weight (Da) 120,316
Isoelectric Point (pI) 5.28
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:534582 to 531307 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3276 534582..531307 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000002446

ENA2 is the second member of a tandem array of genes encoding nearly, but not perfectly, identical P-Type ATPases. The reference strain, S288c, contains three genes in this cluster: ENA1, ENA2, and ENA5. Other Saccharomyces strains typically contain 4 or 5 genes (ENA1-ENA5) (3, 4).

ENA1 is the most well characterized member of this cluster and is thought to encode the primary plasma membrane Na+-ATPase exporter in S. cerevisiae. Ena1p plays a critical role in the detoxification of Na+ ions and in maintaining ion homeostasis, making Ena1p a principal component of the cell's ability to survive high salt or alkaline conditions (5). Ena1p and Ena2p both transport Li+ ions, but with differing efficiencies (3), and have been shown to export K+ ions concomitantly with Na+, at some salt concentrations (6).

Ena1p-Ena5p are closely related to Pmr1p (3), the Golgi membrane Ca2+-ATPase. Mutations in the human homolog of Pmr1p, ATP2C1, cause the acantholytic skin condition Hailey-Hailey disease (7).

Last updated: 2006-10-31 Contact SGD

References cited on this page View Complete Literature Guide for ENA2
1) Haro R, et al.  (1991) A novel P-type ATPase from yeast involved in sodium transport. FEBS Lett 291(2):189-91
2) Garciadeblas B, et al.  (1993) Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in Saccharomyces cerevisiae. Mol Gen Genet 236(2-3):363-8
3) Wieland J, et al.  (1995) The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na+ pump in the yeast plasma membrane. EMBO J 14(16):3870-82
4) Martinez R, et al.  (1991) A PMR2 tandem repeat with a modified C-terminus is located downstream from the KRS1 gene encoding lysyl-tRNA synthetase in Saccharomyces cerevisiae. Mol Gen Genet 227(1):149-54
5) Platara M, et al.  (2006) The Transcriptional Response of the Yeast Na+-ATPase ENA1 Gene to Alkaline Stress Involves Three Main Signaling Pathways. J Biol Chem 281(48):36632-42
6) Benito B, et al.  (2002) Potassium- or sodium-efflux ATPase, a key enzyme in the evolution of fungi. Microbiology 148(Pt 4):933-41
7) Marie Mauro T  (2004) Yeast researchers consider Hailey-Hailey disease. J Invest Dermatol 123(6):xxii-xxiii