NSI1/YDR026C Summary Help

Standard Name NSI1 1
Systematic Name YDR026C
Feature Type ORF, Verified
Description RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description NTS1 SIlencing protein 1 1
Chromosomal Location
ChrIV:494268 to 492556 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NSI1 GO evidence and references
  View Computational GO annotations for NSI1
Molecular Function
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
61 total interaction(s) for 44 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-Western: 3
  • Biochemical Activity: 2
  • Reconstituted Complex: 5
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 18
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 2
  • Positive Genetic: 8
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 570
Molecular Weight (Da) 66,352
Isoelectric Point (pI) 10.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:494268 to 492556 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1713 494268..492556 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002433
References cited on this page View Complete Literature Guide for NSI1
1) Ha CW, et al.  (2012) Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae. Nucleic Acids Res 40(11):4892-903
2) Mohanty BK and Bastia D  (2004) Binding of the replication terminator protein Fob1p to the Ter sites of yeast causes polar fork arrest. J Biol Chem 279(3):1932-41
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Fleischer TC, et al.  (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307
5) Reiter A, et al.  (2012) The Reb1-homologue Ydr026c/Nsi1 is required for efficient RNA polymerase I termination in yeast. EMBO J 31(16):3480-93