Standard Name	NSI1 1
Systematic Name	YDR026C
Feature Type	ORF, Verified
Description	RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	NTS1 SIlencing protein 1 1

Chromosomal Location	ChrIV:494268 to 492556 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for NSI1
Molecular Function
High-throughput	DNA binding (IPI)
Biological Process
Manually curated	chromatin silencing at rDNA (IMP) negative regulation of extrachromosomal rDNA circle accumulation involved in replicative cell aging (IMP) termination of RNA polymerase I transcription (IDA, IMP)
Cellular Component
Manually curated	nucleolus (IDA) rDNA heterochromatin (IDA)
High-throughput	nucleolus (IDA) colocalizes_with ribosome (IDA)

Resources	ScerTF | YeTFaSCo

Classical genetics
null	replicative lifespan: decreased viable
Large-scale survey
null	competitive fitness: decreased resistance to selenomethionine: increased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	61 total interaction(s) for 44 unique genes/features.
Physical Interactions	Affinity Capture-MS: 9 Affinity Capture-Western: 3 Biochemical Activity: 2 Reconstituted Complex: 5 Two-hybrid: 6
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 18 Phenotypic Enhancement: 4 Phenotypic Suppression: 2 Positive Genetic: 8 Synthetic Growth Defect: 2 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrIV:494268 to 492556 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..1713 494268..492556 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000002433