TRP1/YDR007W Summary Help

Standard Name TRP1 1
Systematic Name YDR007W
Feature Type ORF, Verified
Description Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) (2 and see Summary Paragraph)
Name Description TRyPtophan requiring
Chromosomal Location
ChrIV:461842 to 462516 | ORF Map | GBrowse
Genetic position: 1 cM
Gene Ontology Annotations All TRP1 GO evidence and references
  View Computational GO annotations for TRP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Regulators 3 genes
Classical genetics
Large-scale survey
47 total interaction(s) for 36 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 4

Genetic Interactions
  • Dosage Rescue: 7
  • Negative Genetic: 11
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 5
  • Positive Genetic: 3
  • Synthetic Growth Defect: 10
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 224
Molecular Weight (Da) 24,144
Isoelectric Point (pI) 5.16
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrIV:461842 to 462516 | ORF Map | GBrowse
Genetic position: 1 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2004-02-11
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..675 461842..462516 2011-02-03 2004-02-11
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002414

The TRP1 gene encodes phosphoribosylanthranilate isomerase, an enzyme that catalyzes the third step in tryptophan biosynthesis. Trp1p catalyzes the conversion of N-5'-phosphoribosyl-anthranilate to 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (3). Because trp1 mutants require tryptophan to grow, the TRP1 gene has been used as a convenient marker in strain and plasmid construction. In addition to their tryptophan auxotrophy, trp1 mutants are also cold sensitive (4, 5). TRP1 has also been frequently utilized in genetic mapping experiments; it is closely linked to the centromere on chromosome IV (CEN4) (6).

Although, the systematic sequence for TRP1/YDR007W originally contained a nonsense (ochre) mutation at codon 67, it has since been shown that S288C does not contain this mutation in the TRP1 gene. The TRP1 mutation was introduced during the creation of strain AB972. AB972 is an ethidium bromide-induced derivative of the strain X2180-1B-trp1, which was supplied by E. Jones [M.V. Olson]. The lineage of AB972 traces directly to the strain S288C with no intervening outcrosses. The strain AB972 was the origin of the clone used for sequencing this segment of chromosome IV. In order to more accurately present this region of within the S288C reference strain, SGD has sequenced the S288C TRP1 locus and did not find the internal STOP codon. Thus, on Februrary 11, 2004, SGD updated the TRP1 sequence by changing the previously annotated internal STOP (TAA) to serine (TCA).

Last updated: 2000-01-20 Contact SGD

References cited on this page View Complete Literature Guide for TRP1
1) Campbell, D.  (1989) Personal Communication, Mortimer Map Edition 10
2) Braus GH  (1991) Aromatic amino acid biosynthesis in the yeast Saccharomyces cerevisiae: a model system for the regulation of a eukaryotic biosynthetic pathway. Microbiol Rev 55(3):349-70
3) Hommel U, et al.  (1995) Phosphoribosyl anthranilate isomerase catalyzes a reversible amadori reaction. Biochemistry 34(16):5429-39
4) Hampsey M  (1997) A review of phenotypes in Saccharomyces cerevisiae. Yeast 13(12):1099-133
5) Stolz LE, et al.  (1998) INP51, a yeast inositol polyphosphate 5-phosphatase required for phosphatidylinositol 4,5-bisphosphate homeostasis and whose absence confers a cold-resistant phenotype. J Biol Chem 273(19):11852-61
6) Mortimer RK and Hawthorne DC  (1966) Genetic mapping in Saccharomyces. Genetics 53(1):165-73